Microscopy and Dynamic Imaging Unit

Brightfield & other Transmission Microscopy Contrasts

Brightfield, Phase Contrast, Differential Interference Contrast (DIC)

FLIM- Fluorescence Lifetime Imaging Microscopy

FLIM is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent samp...

FRET- Förster Resonance Energy Transfer

Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Reso...

High Speed Laser Scanning Confocal Systems

Resonant scanners

Image Analysis/ Software

In vivo Imaging (animal)

none

Laser Microdissection & Catapulting

Laser-based dissection with cellular resolution, on Histological preparations. This technology can harvest the cells of interest directly or can isol...

Laser Scanning Confocal Microscopy

Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimina...

Live Cell Imaging

Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...

Non-Linear Imaging/ Multiphoton fluorescence Imaging

Multiphoton microscopy uses pulsed long-wavelength light to excite fluorophores within the specimen being observed. The fluorophore absorbs the energy...

Non-Linear Imaging/ SHG-Second Harmonic Generation

In biological and medical science, the effect of second harmonic generation is used for high-resolution optical microscopy. Because of the non-zero se...

Photoactivation PA-FPs

Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...

Photobleaching Techniques/ FRAP-Fluorescence Recovery After...

Photobleaching techniques are used as a strategy to reveal the diffusion/binding dynamics of molecules or the exchange between compartments in live ce...

Photoconversion

Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...

Stereomicroscopy

The stereomicroscope is designed for low magnification observation of a sample. It uses two separate optical paths with two objectives and two eyepiec...

Super Resolution/ Stimulated Emission Depletion microscopy (...

STED (Stimulated Emission Depletion Microscopy) uses two laser pulses, the excitation pulse for excitation of the fluorophores to their fluorescent st...

Super Resolution/ Stochastic Optical Reconstruction microsco...

Stochastic Optical Reconstruction Microscopy (STORM), as well as PALM or fPALM, is a super-resolution imaging technique that utilizes sequential activ...

Total Internal Reflection Fluorescence Microscopy (TIRF)

Critical angle incidence of a laser beam onto the inner surface of a coverglass creates an evanescent field which illuminates the field of view with a...

Wide Field Fluorescence Microscopy

Conventional fluorescence excitation, upright or inverted, incl. Hg lamp, Metal-Halide or LED illumination.

Single Plane Illumination Microscopy (SPIM)

Fluorescence illumination is performed laterally with a laser 'lightsheet', i.e. a plane of light traveling exactly along the image plane, originating...