Equipment
Total Internal Reflection Microscopy (TIRF)
Wide Field Fluorescence Microscope
Leica Microsystems AM TIRF MC / Leica AM TIRF MC Inverted TIRF DMI6000
Inverted TIRF DMI6000
Description
Leica DMI6000 inverted microscope coupled to a Laser TIRF system:
-Laser lines: 488,561 and 635nm
-Objectives: HCX PL APO 20x 0,7 dry HCX PL APO 40x 1,25 oil HCX PL APO 100x 1,46 oil
-2 EM-CCD ANDOR Ixon cameras (DU897 and DU8285 VP)
-Incubation chamber for CO2 and temperature control
-Acquisition software: LAS-AF 2.6.0. build 7266