Description
Nikon Eclipse-Ti inverted microscope coupled to a A1R confocal system:
-Laser lines: 402,457,476,488,514,561 and 642nm
-4 PMT detectors with emission filters + 1 transmission detector
-Scanners: galvano/resonant
-Objectives:
Plan Apo 10x/0,45 DIC L dry Plan Fluor EL WD 20x/0,45 DIC L dry Plan Apo VC 20x/0,75 DIC N2 dry Plan Fluor 40x/1,3 Oil DIC H N2 Oil Plan Apo VC 60x/1,4 Oil DIC N2
-Incubation chamber for CO2 and temperature control
-Acquisition software: NIS Elements AR 3.22.08 725
Applicable Technologies
- High Speed Laser Scanning Confocal Systems
High Speed Laser Scanning Confocal Systems
Resonant scanners
- Laser Scanning Confocal Microscopy
Laser Scanning Confocal Microscopy
Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimina...
- Live Cell Imaging
Live Cell Imaging
Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...
- Photoactivation PA-FPs
Photoactivation PA-FPs
Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...
- Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photoble...
Photobleaching Techniques/ FRAP-Fluorescence Recovery After...
Photobleaching techniques are used as a strategy to reveal the diffusion/binding dynamics of molecules or the exchange between compartments in live ce...
- Photoconversion
Photoconversion
Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...