Description
Zeiss Axiovert 200M inverted microscope coupled to a LSM700 confocal system:
-Laser lines: 405,488, 555 and 639nm
-2 PMT detectors with emission filters + 1 transmission detector
-Objectives:
Plan-Apochromat 10x/0,45 M27 LD LCI Plan-Apochromat 25x/0,8 Imm Korr DIC M27 I LCI Plan-Neofluar 63x/1,3 Imm Korr DIC M27 Plan-Apochromat 40x/1,3 Oil DIC M27 Plan-Apochromat 63x/1,40 Oil DIC M27
-Mini-incubator for CO2 and temperature control
-Acquisition software: ZEN 2011
Applicable Technologies
- FRET- Förster Resonance Energy Transfer
FRET- Förster Resonance Energy Transfer
Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Reso...
- Laser Scanning Confocal Microscopy
Laser Scanning Confocal Microscopy
Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimina...
- Live Cell Imaging
Live Cell Imaging
Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...
- Photoactivation PA-FPs
Photoactivation PA-FPs
Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...
- Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photoble...
Photobleaching Techniques/ FRAP-Fluorescence Recovery After...
Photobleaching techniques are used as a strategy to reveal the diffusion/binding dynamics of molecules or the exchange between compartments in live ce...
- Photoconversion
Photoconversion
Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...