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Technologies

Technologies

Techniques and technologies used in Spanish laboratories.

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179 technologies

Single-Molecule Localization Microscopy (PALM, STORM, PAINT)

Stochastic activation and localization of single molecules over thousands of frames to reconstruct an image with ~20 nm precision.

- AFM- Atomic Force microscopy

Atomic force microscopy (AFM), or scanning force microscopy (SFM), is a very high-resolution type of scanning probe microscopy, with demonstrated resolution on the order of fractions of a nm, more than 1000 times better than the optical diffraction limit. The AFM is one of the foremost tools for ima...

- Bioluminescence Imaging (BLI)

Bioluminescence imaging (BLI) is a technology that allows for the noninvasive study of ongoing biological processes in small laboratory animals. Bioluminescence is the process of light emission in living organisms. Bioluminescence imaging utilizes native light emission from one of several organisms...

- Brightfield & other Transmission Microscopy Contrasts

Brightfield illumination has been one of the most widely used observation modes in optical microscopy for the past 300 years. The technique is best suited for utilization with fixed, stained specimens or other kinds of samples that naturally absorb significant amounts of visible light. Images produc...

- Correlated Light and Electron Microscopy (CLEM)

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. Merging fluorescence and electron microsc...

- FCS- Fluorescence Correlation Spectroscopy

Fluorescence Correlation Spectroscopy (FCS) is a tool that provides quantitative localized measurements of important physical parameters including mechanisms of transport, molecular mobilities, and densities of fluorescently labeled species. In the most basic configuration, FCS examines the inherent...

- FLIM- Fluorescence Lifetime Imaging Microscopy

FLIM is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. It can be used as an imaging technique inconfocal microscopy, two-photon excitation microscopy, and multiphoton tomography. The lifetime of the fl...

- FRET- Förster Resonance Energy Transfer

Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Resonance energy transfer is a distance-dependent phenomenon due to the dipole-dipole interaction between the donor and acceptormolecules. The efficiency...

- Generic

- HCS- High-Content Screening

High-Content Screening (HCS) is a powerful technology which gathers Biology and Engineeringaround hardware development and experimental protocols optimization. All share a commongoal: increasing Imaging-throughput, and common means: state-of-the-art Automation. HCSdevelops around functional Cell-Bi...

- High Speed Laser Scanning Confocal Systems

- Image Analysis

- Image Analysis/ Colocalization

- Image Analysis/ Software

- Image Analysis/Deconvolution

Deconvolution is a computationally intensive image processing technique which is designed to remove or reverse the blurring present in microscope images induced by the limited aperture of the objective.

- Image Scanning Microscopy (ISM)

A 3D imaging technique using detector array and structured illumination to yield super resolution imaging with almost 2x resolution increase and higher sensibility over classical laser scanning confocal microscopy. Implemented in 1) laser scanning confocal microscopes where point detectors are repl...

- In vivo Imaging (animal)

- Labelling/ Fluorescent Proteins

- Labelling/ Fluorophores

- Labelling/ Fluorophores/ Spectra

- Laser Microdissection & Catapulting

Laser-based dissection with cellular resolution, on Histological preparations. This technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. A laser is coupled into a microscope and focu...

- Laser Scanning Confocal Microscopy

Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimination or reduction of background information away from the focal plane (that leads to image degradation), and the capability to collect serial optical...

- Lattice Lightsheet

Lattice light-sheet microscopy is a modified version of lightsheet fluorescence microscopy that increases image acquisition speed and resolution, while decreasing damage to cells caused by phototoxicity. While classical lightsheet microscopy uses a static (SPIM) or scanned (DSLM) ligthsheet illumin...

- Lightsheet, "Open-Top" & single objective variants

While classical lightsheet architectures (e.g. SPIM and variants) use orthogonal lenses to couple illumination and detection, sample mounting through conventional sample carriers (dish, plate, ...) is usually not possible. iSPIM and lattice lightsheet are exceptions, but they requires lenses to dip...