Nikon Eclipse Ti / Nikon Eclipse Ti Time Lapse Inverted

Epifluorescence in vivo time-lapse inverted microscope:

-Objectives:

Plan Fluor 4x/0,13 PhL DL Plan Fluor 10x/0,3 Ph1 DLL S Plan Fluor ELWD 20x/0,45 Ph1 ADM Plan Apo λ 20x/0,75 Plan Fluor ELWD 40x/0.6 Ph2 DM Plan Apo VC 60x/1,4 Oil DIC N2

-Fluorescence filters:

DAPI: Exc 381-392nm; Em 447-507nm GFP: Exc 457-487nm; Em 502-538nm RED: Exc 542-582nm; Em 604-644nm Cy5: Exc 608-648nm; Em 672-712nm

-Cameras:

Hamamatsu CCD ORCA-ER B/W ANDOR sCMOS ZYLA B/W

-Mini-incubator for CO2 and temperature control

-Acquisition software: Nikon ACT-1 v 2.70