Description
Epifluorescence in vivo time-lapse inverted microscope:
-Objectives:
Plan Fluor 4x/0,13 PhL DL Plan Fluor 10x/0,3 Ph1 DLL S Plan Fluor ELWD 20x/0,45 Ph1 ADM Plan Apo λ 20x/0,75 Plan Fluor ELWD 40x/0.6 Ph2 DM Plan Apo VC 60x/1,4 Oil DIC N2
-Fluorescence filters:
DAPI: Exc 381-392nm; Em 447-507nm GFP: Exc 457-487nm; Em 502-538nm RED: Exc 542-582nm; Em 604-644nm Cy5: Exc 608-648nm; Em 672-712nm
-Cameras:
Hamamatsu CCD ORCA-ER B/W ANDOR sCMOS ZYLA B/W
-Mini-incubator for CO2 and temperature control
-Acquisition software: Nikon ACT-1 v 2.70
Applicable Technologies
- Brightfield & other Transmission Microscopy Contrasts
Brightfield & other Transmission Microscopy Contrasts
Brightfield, Phase Contrast, Differential Interference Contrast (DIC)
- Live Cell Imaging
Live Cell Imaging
Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...
- Wide Field Fluorescence Microscopy
Wide Field Fluorescence Microscopy
Conventional fluorescence excitation, upright or inverted, incl. Hg lamp, Metal-Halide or LED illumination.