Description
Axio Examiner Z1 upright microscope coupled to a Confocal/Multiphoton/FLIM system with illumination sources for one and two photon excitation:
-Laser lines: 405,458,488,514,561,633nm Mai Tai DS 690-1040 nm
-3 GASP detectors (1 spectral detector + 2 Becker&Hickl GmbH FLIM detectors in the NDD port) + 2PMTs + 1 Transmission detector.
-Objectives: W Plan-Apochromat 20x/1,0 DIC M27 75m W Plan-Apochromat 40x/1,0 DIC M27 Plan-Apochromat 10x/0,45 M27
-Mini-incubator for CO2 and temperature control
-Acquisition software: ZEN 2011
Applicable Technologies
- FLIM- Fluorescence Lifetime Imaging Microscopy
FLIM- Fluorescence Lifetime Imaging Microscopy
FLIM is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent samp...
- FRET- Förster Resonance Energy Transfer
FRET- Förster Resonance Energy Transfer
Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Reso...
- In vivo Imaging (animal)
In vivo Imaging (animal)
none
- Laser Scanning Confocal Microscopy
Laser Scanning Confocal Microscopy
Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimina...
- Live Cell Imaging
Live Cell Imaging
Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...
- Non-Linear Imaging/ Multiphoton fluorescence Imaging
Non-Linear Imaging/ Multiphoton fluorescence Imaging
Multiphoton microscopy uses pulsed long-wavelength light to excite fluorophores within the specimen being observed. The fluorophore absorbs the energy...
- Non-Linear Imaging/ SHG-Second Harmonic Generation
Non-Linear Imaging/ SHG-Second Harmonic Generation
In biological and medical science, the effect of second harmonic generation is used for high-resolution optical microscopy. Because of the non-zero se...
- Photoactivation PA-FPs
Photoactivation PA-FPs
Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...
- Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photoble...
Photobleaching Techniques/ FRAP-Fluorescence Recovery After...
Photobleaching techniques are used as a strategy to reveal the diffusion/binding dynamics of molecules or the exchange between compartments in live ce...
- Photoconversion
Photoconversion
Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...