Fluorescence lifetime imaging microscopy (FLIM) — single-photon or multiphoton Micro@CNIC Madrid

FLIM- Fluorescence Lifetime Imaging Microscopy

About the Installation

Measures the fluorescence decay rate, providing information on the molecular microenvironment (pH, Ca2+, quenching) independent of intensity.

How this lab uses this technology

We train internal and external to the use of FLIM and phasor analysis to study cellular metabolism, endogenous autofluorescence, drug disposition, and tissue physiology in live cells and biological specimens, providing sensitive and non-invasive functional information at the microscopic level. We welcome collaborative projects on FLIM applications.

Lab-specific experience

Started in 2008, our present FLIM platform is based on three confocal-2photon microscopes that combines time-correlated single-photon counting (TCSPC), white laser multicolor FLIM and phasor analysis with confocal imaging.

Equipment

Zeiss (Carl Zeiss Microscopy Gmbh) Multiphoton / Zeiss Multiphoton Multiphoton – FLIM - LSM Confocal Zeiss LSM 780

Multiphoton – FLIM - LSM Confocal Zeiss LSM 780

Zeiss (Carl Zeiss Microscopy Gmbh) · Multiphoton

Nikon Eclipse Ti / Nikon Eclipse Ti CUSTOM FLIM and Multiphoton Nikon-inverted ISS-ALBA

CUSTOM FLIM and Multiphoton Nikon-inverted ISS-ALBA

Nikon · Eclipse Ti

Leica Microsystems STELLARIS 8 FALCON CRS / Leica 4TuneDualIRDIVE STELLARIS 8 FALCON

https://www.cnic.es/es/investigacion/microscopia/equipos/leica-4tunedualirdive-stellaris-8-falcon-ayuda-eqc2021-007527-p

Leica Microsystems · STELLARIS 8 FALCON CRS

Notes

Caiolfa, Valeria R., Moreno Zamai, Gabriele Malengo, Annapaola Andolfo, Chris D. Madsen, Jason Sutin, Michelle A. Digman, Enrico Gratton, Francesco Blasi, and Nicolai Sidenius. 2007. Journal of Cell Biology, 179: 1067-82. Digman, Michelle A., Valeria R. Caiolfa, Moreno Zamai, and Enrico Gratton. 2008. Biophysical Journal, 94: L14-L16. Ossato, Giulia, Moreno Zamai, Alberto Rosello-Diez, Miguel Torres, and Valeria Rosaria Caiolfa. 2012. Biophysical Journal, 102: 193A-93A. Rocha-Perugini, V., M. Zamai, J. M. Gonzalez-Granado, O. Barreiro, E. Tejera, M. Yanez-Mo, V. R. Caiolfa, and F. Sanchez-Madrid. 2013. Molecular and Cellular Biology, 33: 3644-58. Sampietro, Marta, Moreno Zamai, Alfonsa Diaz Torres, Veronica Labrador Cantarero, Federica Barbaglio, Lydia Scarfo, Cristina Scielzo, and Valeria R. Caiolfa. 2021. Frontiers in Cell and Developmental Biology, 9. Zamai, M., A. Andolfo, R. Fadin, M. Collini, L. D'Alfonso, F. Blasi, N. Sidenius, and V. R. Caiolfa. 2005. Biophysical Journal, 88: 255A-55A.