Description
Leica DMI6000B inverted microscope coupled to a confocal system:
-Laser lines: 405,458,476,488,496,514,561,594 and 633nm
-5 spectral fluorescence detectors + 1 transmission detector
-Scanners: galvano/resonant
-Objectives:
HCX PL APO CS 10x 0,4 dry HCX PL APO lambda blue 20x 0,7 multi-imm HCX PL APO CS 40x 1,25 oil HCX PL APO lambda blue 63x 1,2 water HCX PL APO CS 63 x 1,3 glycerol HCX PL APO lambda blue 63x 1,40 oil
-Incubation chamber for CO2 and temperature control
-Acquisition software: LAS-AF 2.6.0. build 7266
Applicable Technologies
- FRET- Förster Resonance Energy Transfer
FRET- Förster Resonance Energy Transfer
Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Reso...
- High Speed Laser Scanning Confocal Systems
High Speed Laser Scanning Confocal Systems
Resonant scanners
- Laser Scanning Confocal Microscopy
Laser Scanning Confocal Microscopy
Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimina...
- Live Cell Imaging
Live Cell Imaging
Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...
- Photoactivation PA-FPs
Photoactivation PA-FPs
Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...
- Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photoble...
Photobleaching Techniques/ FRAP-Fluorescence Recovery After...
Photobleaching techniques are used as a strategy to reveal the diffusion/binding dynamics of molecules or the exchange between compartments in live ce...
- Photoconversion
Photoconversion
Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...