Description
Customized Nikon Eclipse Ti inverted microscope coupled to a Multiphoton and FLIM system:
-MaiTai DS Laser. 690-1040 nm.
-2 PMT+ 2 APD detectors (1 ISS-fast-FLIM + 1 ISS- Fluorescence Correlation Spectroscopy)
-Objectives: Dry (40X/0.9; 40X/0.6) Water immersion (60X/1.2) Oil immersion (40X/0.9; 60X/1.2)
-Mini-incubator for CO2 and temperature control
-Acquisition software: VistaVision 4.0.119
Applicable Technologies
- FLIM- Fluorescence Lifetime Imaging Microscopy
FLIM- Fluorescence Lifetime Imaging Microscopy
FLIM is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent samp...
- Live Cell Imaging
Live Cell Imaging
Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...
- Non-Linear Imaging/ Multiphoton fluorescence Imaging
Non-Linear Imaging/ Multiphoton fluorescence Imaging
Multiphoton microscopy uses pulsed long-wavelength light to excite fluorophores within the specimen being observed. The fluorophore absorbs the energy...