Super Resolution/ Stochastic Optical Reconstruction microscopy (STORM)

Stochastic Optical Reconstruction Microscopy (STORM), as well as PALM or fPALM, is a super-resolution imaging technique that utilizes sequential activation and time-resolved localization of photoswitchable fluorophores to create high resolution images. During imaging, only an optically resolvable subset of fluorophores is activated to a fluorescent state at any given moment, such that the position of each fluorophore can be determined with high precision by finding the centroid position of the single-molecule images. The fluorophore is subsequently deactivated, and another subset is activated and imaged. Iteration of this process allows numerous fluorophores to be localized and a super-resolution image to be constructed from the image data. STORM has been demonstrated with a variety of different probes and labeling strategies.