Advanced Optical Microscopy Service - Nanoimaging Unit

Bioluminescence Imaging (BLI)

Bioluminescence imaging (BLI) is a technology that allows for the noninvasive study of ongoing biological processes in small laboratory animals. Biolu...

Brightfield & other Transmission Microscopy Contrasts

Brightfield illumination has been one of the most widely used observation modes in optical microscopy for the past 300 years. The technique is best su...

FRET- Förster Resonance Energy Transfer

Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Reso...

HCS- High-Content Screening

High-Content Screening (HCS) is a powerful technology which gathers Biology and Engineeringaround hardware development and experimental protocols opti...

High Speed Laser Scanning Confocal Systems

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In vivo Imaging (animal)

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Laser Scanning Confocal Microscopy

Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimina...

Live Cell Imaging

Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...

Image Analysis

Image Analysis/ Colocalization

Image Analysis/ Software

Labelling/ Fluorescent Proteins

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Photobleaching Techniques/ FLIP-Fluorescence Loss In Photobl...

This technique is used to study the diffusion of molecules among different connected/communicated cell compartments. A fluorescent cell is repeatedly...

Photobleaching Techniques/ FRAP-Fluorescence Recovery After...

Photobleaching techniques are used as a strategy to reveal the diffusion/binding dynamics of molecules or the exchange between compartments in live ce...

Photoconversion

Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...

Spinning Disk Confocal Microscopy

Laser illumination and sample's emission lights travel through a revolving Mask made of pinholes (e.g. Nipkow disk) to provide optical sectioning. Bec...

Super Resolution/ Stochastic Optical Reconstruction microsco...

Stochastic Optical Reconstruction Microscopy (STORM), as well as PALM or fPALM, is a super-resolution imaging technique that utilizes sequential activ...

Total Internal Reflection Fluorescence Microscopy (TIRF)

Critical angle incidence of a laser beam onto the inner surface of a coverglass creates an evanescent field which illuminates the field of view with a...

Wide Field Fluorescence Microscopy

Conventional fluorescence excitation, upright or inverted, incl. Hg lamp, Metal-Halide or LED illumination.

Stereomicroscopy

The stereomicroscope is designed for low magnification observation of a sample. It uses two separate optical paths with two objectives and two eyepiec...