Advanced Digital Microscopy Core Facility (IRB Barcelona)

HCS- High-Content Screening

High Content Imaging/Analysis (HCI/HCA), and Screening (HCS/HTS) with Confocal and Widefield systems. Smart imaging, multiwell plates loading for HTS...

Total Internal Reflection Fluorescence Microscopy (TIRF)

Total Internal Reflection Microscopy. 2-colors applications for membrane dynamics, cytoskeleton, in vitro studies, bio-functionalized surfaces.

Live Ablation and Pulsed Laser Micro- & Nano-Surgery

Pulsed-laser Micro- and Nano-Surgery in living cells and organisms. Applications: Intracellular surgery: Cytoskeletal dynamics, organelle ablation a...

Single Plane Illumination Microscopy (SPIM)

= Custom Development of 2 Light-sheet systems: Live Imaging of Large samples (e.g. embryos, organoids...) Full Organ Imaging with optically cleared s...

Image Analysis

Custom Analysis Development in Java, Matlab, ImageJ-related programming languages. Software used: ImageJ / Fiji, Matlab, Imaris, Ilastik, Amira, NI Vi...

FLIM- Fluorescence Lifetime Imaging Microscopy

FLIM is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent samp...

FRET- Förster Resonance Energy Transfer

Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Reso...

Lightsheet, "Open-Top" & single objective variants

While classical lightsheet architectures (e.g. SPIM and variants) use orthogonal lenses to couple illumination and detection, sample mounting through...

Super Resolution/ Stimulated Emission Depletion microscopy (...

STED (Stimulated Emission Depletion Microscopy) uses two laser pulses, the excitation pulse for excitation of the fluorophores to their fluorescent st...

FCS- Fluorescence Correlation Spectroscopy

Fluorescence Correlation Spectroscopy (FCS) is a tool that provides quantitative localized measurements of important physical parameters including mec...

Super Resolution/ Stochastic Optical Reconstruction microsco...

Stochastic Optical Reconstruction Microscopy (STORM), as well as PALM or fPALM, is a super-resolution imaging technique that utilizes sequential activ...

Super Resolution/ Structured Illumination Microscopy (SIM)

Structured Illumination (SI) - or patterned illumination - is the wide-field version of breaking the diffraction limit of light. SI relies on both spe...

Image Scanning Microscopy (ISM)

A 3D imaging technique using detector array and structured illumination to yield super resolution imaging with almost 2x resolution increase and highe...

Spinning Disk Confocal Microscopy

Laser illumination and sample's emission lights travel through a revolving Mask made of pinholes (e.g. Nipkow disk) to provide optical sectioning. Bec...

Laser Scanning Confocal Microscopy

Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimina...

Live Cell Imaging

Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...

Wide Field Fluorescence Microscopy

Conventional fluorescence excitation, upright or inverted, incl. Hg lamp, Metal-Halide or LED illumination.