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Total Internal Reflection Fluorescence Microscopy (TIRF)

Total Internal Reflection Fluorescence (TIRF, widefield variant) Servicio de Microscopia Optica Avanzada del Centro Nacional de Biotecnologia Madrid
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Equipment
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Technical Resources

Description

Utilizes an evanescent field (100–200 nm depth) to selectively excite fluorophores near the coverglass, drastically reducing background noise.

How this lab uses this technology

Utilizes an evanescent field (100–200 nm depth) to selectively excite fluorophores near the coverglass, drastically reducing background noise.

Equipment

Leica Microsystems DMi8 fluorescence and TIRF/SMLM system / Leica Micr...

YODA

Leica Microsystems DMi8 fluorescence and TIRF/SMLM system Total Internal Reflection Microscopy (TIRF) Wide Field Fluorescence Microscope

Abbelight SAS SAFe 360

Abbelight SAS SAFe 360