Technology
Total Internal Reflection Fluorescence (TIRF, widefield variant)
About this Technology
Utilizes an evanescent field (100–200 nm depth) to selectively excite fluorophores near the coverglass, drastically reducing background noise.
Labs Using this Technology
SMOA
Servicio de Microscopia Optica Avanzada del Centro Nacional de Biotecnologia
Total Internal Reflection Fluorescence Microscopy (TIRF)
Consejo Superior de Investigaciones Cientificas (CSIC) · Madrid
Unidad de Microscopía
Centro Andaluz de Biología Molecular y Medicina Regenerativa CABIMER · Sevilla