Applicable Technologies
Total Internal Reflection Fluorescence (TIRF, widefield variant)
Total Internal Reflection Fluorescence Microscopy (TIRF)
Utilizes an evanescent field (100–200 nm depth) to selectively excite fluorophores near the coverglass, drastically reducing background noise.
Single-Molecule Localization Microscopy (PALM, STORM, PAINT)
Super Resolution/ Stochastic Optical Reconstruction microsco...
Stochastic activation and localization of single molecules over thousands of frames to reconstruct an image with ~20 nm precision.
Single Particle Tracking (SPT)
Single Particle Tracking (SPT)
High-speed imaging and localization of individual molecules or organelles to study their trajectories and diffusion modes in live cells.