Description
Orca Flash 4.0 sCMOS camera (Hamamatsu).
TIRFM (Total Internal Reflection Fluorescence Microscopy) module.
High-power 405, 488, 561 and 638 nm lasers for Single-Molecule Localization Microscopy (STORM/PALM).
Gemini W-View splitter (Hamamatsu) for simultaneous acquisition with green/red fluorochromes.
Environmental control camera (Okolab) for time-lapse studies.
Applicable Technologies
Widefield Fluorescence Microscopy (WFF)
Wide Field Fluorescence Microscopy
Conventional fluorescence imaging using broadband sources (LED, Metal-Halide, or Hg lamps) for full-field excitation.
Brightfield Microscopy (BFM)
Brightfield & other Transmission Microscopy Contrasts
Basic trans-illumination where contrast arises from light absorption by the specimen; typically used for fixed, stained tissues.
Total Internal Reflection Fluorescence (TIRF, widefield variant)
Total Internal Reflection Fluorescence Microscopy (TIRF)
Utilizes an evanescent field (100–200 nm depth) to selectively excite fluorophores near the coverglass, drastically reducing background noise.
Single-Molecule Localization Microscopy (PALM, STORM, PAINT)
Super Resolution/ Stochastic Optical Reconstruction microsco...
Stochastic activation and localization of single molecules over thousands of frames to reconstruct an image with ~20 nm precision.