Description
A sub-nanosecond pulsed UV laser performing submicron ablation in cells and embryos, coupled onto an inverted microscope equipped with environmental control and spinning disk. Combination with FRAP also possible.
Multiscale performance: DNA Damage, subcellular ablation, organelles ablation, single cell ablation, cell scission, multicellular ablation, organ ablation, etc...
Applications: DNA damage kinetics, Cell biophysics, biomechanics, regeneration, morphogenesis, wound healing, tissue mechanics and development, micromanipulation, correlative microscopy LM-EM (laser etching to find samples) etc...
Additional Details
Laser specs: 355nm tripled Nd:YAG, pulse duration 470ps, rep rate up to 1kHz. Fluorescence imaging through a Nipkow spinning disk, with 3 lasers 488, 561, 640nm.
Applicable Technologies
- Spinning Disk Confocal Microscopy
Spinning Disk Confocal Microscopy
Laser illumination and sample's emission lights travel through a revolving Mask made of pinholes (e.g. Nipkow disk) to provide optical sectioning. Bec...
- Live Cell Imaging
Live Cell Imaging
Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...
- Wide Field Fluorescence Microscopy
Wide Field Fluorescence Microscopy
Conventional fluorescence excitation, upright or inverted, incl. Hg lamp, Metal-Halide or LED illumination.