Equipment
Laser Microdissection & Catapulting
Spinning Disk Confocal Microscope
Wide Field Fluorescence Microscope
CUSTOM Laser Nanosurgery / CUSTOM Laser Nanosurgery Laser Cutter/Nanosurgery for live specimens
Laser Cutter/Nanosurgery for live specimens
Description
A sub-nanosecond pulsed UV laser performing submicron ablation in cells and embryos, coupled onto an inverted microscope equipped with environmental control and spinning disk. Combination with FRAP also possible.
Multiscale performance: DNA Damage, subcellular ablation, organelles ablation, single cell ablation, cell scission, multicellular ablation, organ ablation, etc...
Applications: DNA damage kinetics, Cell biophysics, biomechanics, regeneration, morphogenesis, wound healing, tissue mechanics and development, micromanipulation, correlative microscopy LM-EM (laser etching to find samples) etc...
Additional Details
Most of experiments performed at 63x and 40x, water immersion NA = 1.2 lenses.
Laser specs: 355nm tripled Nd:YAG, pulse duration 470ps, rep rate up to 1kHz. Fluorescence imaging through a Nipkow spinning disk, with 3 lasers 488, 561, 640nm.
Laser specs: 355nm tripled Nd:YAG, pulse duration 470ps, rep rate up to 1kHz. Fluorescence imaging through a Nipkow spinning disk, with 3 lasers 488, 561, 640nm.