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CUSTOM SPIM / CUSTOM SPIM SPIM for high resolution Live Imaging

SPIM for high resolution Live Imaging

CUSTOM SPIM

Description

Lightsheet Microscope for long-term and multi-view Imaging of large specimens. Typical Applications: embryo development, 3D cell cultures, intravital microscopy, transparent organisms imaging, cultured cells on glass coverslip to be imaged "fast" and at different angles.

Additional Details

sCMOS Hamamatsu Fusion camera.
Fast piezo scan for the z-axis.
ASLM (Axially-Swept Lightsheet) for high axial resolution at low magnification,
DSLM (Digitally Swept Lightsheet) for high contrast,
Refocussing for fast volumetric imaging without sample motion,
Double sided lightsheet illumination.

Imaging of samples embedded in Agarose blocks or on coverslip.
Large chamber with medium circulation.
Double sided illumination, Pivot-scanning under construction.
Lasers: 488nm, 561nm, 638nm.
Water dipping objective lenses: 10x/0.3W, 20x/0.5W, 25x/1.1W, 40x/0.8W, 63x/0.9W, 100x/1.1W.

Applicable Technologies