CUSTOM Macro-SPIM / CUSTOM Macro-SPIM MacroSPIM for full organ imaging

Lightsheet based Macroscope to image full organ and tumors after optical clearing. The system is based on Low/Intermediate magnification Imaging (macroscope) and achieves isotropic detection within a zoom range of 1x-24x. Image tiling working well to generate up to 20x20x3000 images of large organs at higher magnification for single cell detection in organs.

The system offers a unique combination of double illumination ("right" and "left" lightsheets) and double sided detection (2 macroscopes and cameras face-to-face), for deeper imaging.

Currently compatible with the any clearing techniques, as it corrects for any refractive index. Those include the following (tested in the lab since 2011): Dehydration + BABB, Dehydration + DBE, iDisco, 3Disco, CUBIC (several variations), Rapiclear, ECI and ECI2, DeepCLEAR, CLARITY, SeeDB, and more.

Optical zoom from 1.2x to 24x, with front lens 2x, 4x, 5x and internal Zoom up to 8x.
Lightsheet thickness from 4 to 20 microns.
Flash 4 cameras, Hamamatsu.
Lasers: 488, 561, 638nm.
Full Multiposition automation, custom Labview control.
Smart imaging capabilities.
Scattered Lightsheet modality for label free imaging in cleared organs.