Servicio Científico-Técnico de Microscopia e Imagen

Brightfield illumination has been one of the most widely used observation modes in optical microscopy for the past 300 years. The technique is best su...

Conventional fluorescence imaging using broadband sources (LED, Metal-Halide, or Hg lamps) for full-field excitation.

Employs a pinhole aperture and focused laser beams to collect serial optical sections, allowing for high-contrast 3D reconstruction.

An area-detector scanning method that improves resolution by 1.7x and increases signal-to-noise ratio via pixel reassignment.

Uses patterned illumination and computational frequency-space restoration to double the resolution limit (approx. 100–120 nm).

Stochastic activation and localization of single molecules over thousands of frames to reconstruct an image with ~20 nm precision.

Utilizes an evanescent field (100–200 nm depth) to selectively excite fluorophores near the coverglass, drastically reducing background noise.

Uses a revolving disk with multiple pinholes to scan the sample in parallel, enabling high-speed imaging with minimal phototoxicity.

Employs pulsed NIR light for simultaneous two-photon absorption, providing deep tissue penetration and inherent optical sectioning.

Label-free process where non-centrosymmetric structures (e.g., collagen) emit frequency-doubled light without needing fluorophores.

Measures the fluorescence decay rate, providing information on the molecular microenvironment (pH, Ca2+, quenching) independent of intensity.

Photobleaching techniques used to measure the mobility, diffusion coefficients, and exchange rates of tagged molecules.

Fluorescence microscopy technique used to study molecular mobility and intracellular connectivity by monitoring fluorescence depletion over time.

Uses a laser beam to precisely isolate and harvest specific cells or tissue regions from sections for genomic or proteomic analysis.

Computational extraction of quantitative biological parameters (size, intensity, shape) from raw image data.