Description
The Leica AF6000 LX is a fully integrated workstation for high-end widefield fluorescence and live cell imaging. It is specifically engineered to maintain cells under near-physiological conditions, enabling long-term time-lapse experiments with high temporal resolution and minimal phototoxicity.
Key Features & Experimental Capabilities
Live Cell Excellence: Fully enclosed XL Incubation Chamber with precise control of temperature (22°C to 37°C), CO2, and humidity.
Advanced Fluidics: Integrated Gilson MINIPULS® 3 Perfusion System (4-channel peristaltic pump), supporting flow rates from 0.3 µL/min to 220 mL/min for dynamic media exchange or shear stress experiments.
High-Sensitivity Detection: Equipped with the Leica K8 sCMOS camera, featuring high quantum efficiency and low noise for sharp fluorescence signals against dark backgrounds—ideal for dim samples and rapid acquisition.
Fast Acquisition: High-speed filter wheel combined with a triple dichroic block for synchronized multi-channel imaging with minimal mechanical vibration.
Applications
Long-term live cell time-lapse imaging.
Cell dynamics and protein trafficking.
Perfusion and controlled media exchange assays.
High-sensitivity fluorescence quantification.
Lenses / Objectives
- Glycerol Immersion: 63x/1.30 (Ph/DIC), specially corrected for 37°C experiments.
- Oil Immersion: 100x/1.40 (Ph/DIC) for high-resolution imaging.
Additional Details
-UV (A4): Ex 360/40 - Em 470/40.
- Green (L5): Ex 480/40 - Em 527/30.
- Red (N3): Ex 546/12 - Em 600/40.
- Far Red (Y5): Ex 620/60 - Em 700/75.
- High-speed Triple Block: UV (387/11), Blue (494/20), and Yellow (575/25).