Evident (ex-Olympus) IX81 / Inverted Microscope

The Olympus IX81 is a versatile, fully automated inverted fluorescence microscope system designed for high-quality imaging of both fixed samples and live cell cultures. Equipped with a motorized stage and a high-resolution 12MP camera, it is the ideal "workhorse" for researchers requiring automated multi-position imaging and reliable fluorescence documentation.

Key Features & Capabilities

• Advanced Automation: Fully motorized stage controlled by cellSens Dimension software, including the Multi-Position module. This allows for automated screening of multi-well plates, large area stitching (mosaics), and unattended data acquisition at multiple points of interest.

• High-Resolution Imaging: Features an Olympus DP75 (12MP) digital camera, providing excellent color fidelity and sensitivity for complex fluorescence and brightfield applications.

• Optical Versatility: Inverted configuration compatible with standard culture dishes, multi-well plates, and microscope slides. Supports Brightfield (BF), Phase Contrast (Ph), and Fluorescence imaging.

Technical Specifications

• Light Source: Metal Halide fluorescence lamp.

• Fluorescence Filter Sets:

  • UV / Blue (DAPI/Hoechst): Ex 360-370 / Em 420-461 nm.

  • Green (GFP/FITC/Alexa 488): Ex 470-495 / Em 510-550 nm.

  • Red (Cy3/TRITC/Alexa 546-568): Ex 540-550 / Em 575-625 nm.

  • Far Red (Cy5/Alexa 647): Ex 625-655 / Em 665-715 nm.

Applications

• Multi-channel fluorescence imaging (up to 4 colors).

• Automated multi-well plate screening and multi-point time-lapse.

• Morphological analysis in animal and plant tissues.

• Phase contrast imaging for unstained cell cultures.

- 4x/0,13 air (DT 17 mm); BF, Fluo
- 10x/0,3 air (DT 10 mm); BF, Ph, Fluo
- 20x/0,45 air (DT 6,4-7,6 mm); BF, Ph, Fluo
- 40x/0,60 air (DT 2,7-4,0 mm); BF, Ph, Fluo
- 60x/0,65-1,25 oil immersion (DT 0,2mm); BF, Ph, Fluo

· Light Source: Metal Halide fluorescence lamp.

· Fluorescence Filter Sets:
- UV / Blue (DAPI/Hoechst): Ex 360-370 / Em 420-461 nm.
- Green (GFP/FITC/Alexa 488): Ex 470-495 / Em 510-550 nm.
- Red (Cy3/TRITC/Alexa 546-568): Ex 540-550 / Em 575-625 nm.
- Far Red (Cy5/Alexa 647): Ex 625-655 / Em 665-715 nm.