Description
The Zeiss LSM 880 is a spectral laser scanning confocal microscope optimized for high-speed, high-sensitivity imaging. The system is equipped with the Airyscan detector, which surpasses the diffraction limit of standard confocal systems to provide enhanced spatial resolution.
Key Features & Capabilities
Super-Resolution Performance: Achieves up to 140 nm lateral and 400 nm axial resolution.
Spectral & NIR Flexibility: Features a 32-channel GaAsP Quasar detector for spectral unmixing and a NIR Detector (2 channels) with near infrared optimized GaAsP and GaAs detectors specifically filtered for high-sensitivity near-infrared (NIR) detection.
High-Speed Imaging: Fast Airyscan mode enables acquisition at up to 27 frames per second, ideal for rapid physiological processes.
Advanced Environment Control: Full XL incubation system including temperature, Co2, and O2 (Hypoxia) regulation.
Technical Specifications
Lasers: 405nm; Ar-multiline (458, 488, 514nm); 561nm; 633nm; and 730nm.
Detectors: 32-element GaAsP spectral array , 2 PMTs, 1 Airyscan , 2 GaAs/P for NIR , and 1 Transmitted Light PMT.
Automation: Fully motorized XYZ stage with Piezo Z-insert for high-precision Z-stacking.
Applications
Super-resolution 3D/4D reconstruction and animation.
Spectral scanning and unmixing of complex multi-color samples.
Time-lapse imaging under physiological or hypoxic conditions.
Advanced photo-manipulation: FRAP, FRET, FLIP
Lenses / Objectives
- Air: 10x/0,45 y 20x/0,8.
- Oil immersion: 40x/1,3; 63x/1,4 y 100x/1,40.
- Glycerol / Multi-immersion: 25x/0,8 (Multi: water/oil/glycerol) and 63x/1,3 (Glycerol-corrected lense for 37°C).
Applicable Technologies
Laser Scanning Confocal Microscopy (LSCM)
Employs a pinhole aperture and focused laser beams to collect serial optical sections, allowing for high-contrast 3D reconstruction.
Airyscan Confocal Microscopy
An area-detector scanning method that improves resolution by 1.7x and increases signal-to-noise ratio via pixel reassignment.
Fluorescence Recovery After Photobleaching (FRAP)
Photobleaching techniques used to measure the mobility, diffusion coefficients, and exchange rates of tagged molecules.
Fluorescence Loss in Photobleaching (FLIP)
Fluorescence microscopy technique used to study molecular mobility and intracellular connectivity by monitoring fluorescence depletion over time.