Vibratome and Cryostat Sectioning

This training activity provides theoretical and hands-on instruction in the preparation and sectioning of biological samples using vibratomes and cryostats.

Participants learn how to select the most appropriate sectioning method according to the nature of the sample, the required section thickness and the subsequent microscopy or staining technique.

Vibratome Sectioning

Vibratome sectioning is used to obtain relatively thick sections from fresh, fixed or lightly embedded biological samples. Since the technique does not usually require paraffin or resin embedding, it can help preserve tissue morphology, enzymatic activity, fluorescence and antigenicity.

The training covers:

• Sample fixation and preparation

• Orientation and mounting of samples

• Selection and installation of the appropriate blade

• Adjustment of cutting speed, amplitude and section thickness

• Sectioning of plant and animal tissues

• Collection and handling of free-floating sections

• Prevention of tearing, compression and vibration artefacts

• Preparation of sections for histological staining, fluorescence microscopy and immunolocalization

Cryostat Sectioning

Cryostat sectioning is used to obtain thin sections from rapidly frozen biological samples. This method is particularly suitable when the preservation of lipids, enzymatic activity, fluorescence or antigenicity is required.

The training includes:

• Sample collection and rapid freezing

• Use of suitable embedding media for frozen samples

• Orientation of samples in cryostat moulds

• Temperature adjustment according to tissue characteristics

• Preparation and trimming of frozen blocks

• Sectioning and collection of cryosections on microscope slides

• Prevention of folding, cracking and freezing artefacts

• Storage and preparation of sections for staining, fluorescence microscopy and immunohistochemistry

At the end of the activity, participants will be able to select appropriate cutting conditions, obtain sections of suitable quality and identify the most common artefacts associated with vibratome and cryostat sectioning.