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CTEM - Servicio de Microscopía Confocal y Electrónica de Transmisión de la EEZ. CSIC

CTEM - Servicio de Microscopía Confocal y Electrónica de Transmisión de la EEZ. CSIC

CTEM - microscopy core facility

Consejo Superior de Investigaciones Cientificas (CSIC)
Estación Experimental del Zaidín (EEZ-CSIC)
Granada
13
Equipment
29
Technologies
5
Training

Equipment

Leica Microsystems Platform Leica STELLARIS

Leica Microsystems Platform Leica STELLARIS

JEOL Microscopía Electrónica de Transmisión JEM-1011

JEOL Microscopía Electrónica de Transmisión JEM-1011

Nikon Eclipse TE-2000 / Microscopía de barrido laser confocal Nikon C-...

Nikon Eclipse TE-2000

Leica Microsystems DMI6000B / Microscopio Invertido de Epifluorescenci...

Leica Microsystems DMI6000B

Leica Microsystems M165FC / Estereomicroscopio Epifluorescencia

Leica Microsystems M165FC

Leica Microsystems RM 2165 / Microtomo

Leica Microsystems RM 2165

Reichert-Jung ULTRACUT / Ultramicrotomo

Reichert-Jung ULTRACUT

Leica Microsystems EM KMR3 / Máquina de fabricar cuchillas de vidrio

Leica Microsystems EM KMR3

Leica Microsystems VT1200 / VT1200S / Vibratomo

Leica Microsystems VT1200 / VT1200S

Leica Microsystems EM TP / Procesador de tejidos automatizado

Leica Microsystems EM TP

Leica Microsystems EG1150 H / Centro de inclusión de tejidos en parafi...

Leica Microsystems EG1150 H

Open Source Software Workstation / PROCESSING AND ANALYSIS OF IMAGES

Open Source Software Workstation

Leica Microsystems Reichert-Jung/Leica Frigocut 2800 E / Jung Frigocut...

Leica Microsystems Reichert-Jung/Leica Frigocut 2800 E

Available Technologies

Transmission Electron Microscopy (TEM)

TEM FOR PLANTS, MICROBIAL AND ANIMAL TISSUES

PREPARATION TECHNIQUES Transmission electron microscopy (TEM) operates under high-vacuum conditions and requires ultrathin specimens. Biological samp...

Lab-specific experience

CTEM Expertise The CTEM service has extensive experience and expertise in the fixation, processing, resin embedding and ultrathin sectioning of plant, microbial and animal samples. For advice on sample preparation or the selection of the most appropriate protocol for your study, please contact the CTEM technical staff.

- Image Analysis/ Software

Software available

ImageJ/FIJI (https://imagej.net/) Nikon EZ-C1 3.90 FreeViewer (https://nikon-ez-c1-freeviewer.software.informer.com/3.9/) LAS X (https://www.leica-m...

M. Others

Vibratome Sectioning

Vibratome sectioning is a technique used to obtain relatively thick sections of fresh, fixed or lightly processed biological samples without the need...

Lab-specific experience

CTEM Expertise CTEM provides support for vibratome sectioning of plant and animal tissues and for the preparation of thick sections for light, fluorescence, confocal and correlative microscopy applications. Users are encouraged to contact the CTEM technical staff before sample collection or fixation to determine the most appropriate section thickness, fixation conditions and downstream imaging strategy.

Brightfield Microscopy (BFM)

Microtome Sectioning

Microtome sectioning is used to obtain thin sections of biological samples for examination by light microscopy. Depending on the specimen and the expe...

Lab-specific experience

CTEM Expertise CTEM provides support for the preparation and sectioning of plant and animal tissues for light-microscopy applications. Users are encouraged to contact the CTEM technical staff before sample collection or fixation to determine the most appropriate embedding medium, section thickness, staining method and imaging strategy for their study.

Transmission Electron Microscopy (TEM)

Ultrathin Sectioning

Ultrathin sectioning is a precision technique used to obtain electron-transparent sections of resin-embedded samples for examination by transmission e...

Lab-specific experience

CTEM Expertise CTEM has extensive experience in the trimming, ultrathin sectioning and preparation of resin-embedded plant, animal and microbial samples for TEM analysis. The service provides support for: Selection of regions of interest Semithin sectioning before TEM preparation Ultrathin sectioning using glass or diamond knives Collection of sections on TEM grids Contrast enhancement of ultrathin sections Optimization of sectioning conditions for difficult or heterogeneous samples Users are encouraged to contact the CTEM technical staff before sample collection or fixation to determine the most appropriate preparation, embedding and sectioning protocol for their study.

M. Others

Cryostat Sectioning

Cryostat Sectioning Cryostat sectioning is used to obtain thin sections of frozen biological specimens for examination by light, fluorescence or conf...

Lab-specific experience

CTEM Expertise CTEM provides support for the preparation and cryostat sectioning of different biological specimens using the Leica Jung Frigocut 2800. The service includes: Advice on sample collection, fixation and freezing Selection of appropriate cryoprotection conditions Orientation and mounting of specimens Preparation of frozen sections Collection of sections on microscope slides Optimization of cutting temperature and section thickness Preparation of sections for histological, fluorescence and confocal microscopy applications Users should contact the CTEM technical staff before collecting, fixing or freezing samples to determine the most appropriate preparation and sectioning protocol.

Transmission Electron Microscopy (TEM)

Contrast Staining of Grids for Electron Microscopy

Contrast staining of grids is a fundamental step in the preparation of ultrathin sections for transmission electron microscopy (TEM). Its purpose is t...

Lab-specific experience

CTEM Expertise CTEM has experience in the contrast staining of grids containing ultrathin sections from plant, animal and microbial samples for subsequent TEM analysis. The service provides support in: Selection of the most appropriate staining protocol Staining with uranyl acetate and lead citrate Adaptation of the procedure to the sample type and embedding resin Processing of conventional and immunolabelled grids Prevention of precipitates and staining artefacts Evaluation of section quality before TEM observation Final preparation of grids for transmission electron microscopy Users are encouraged to contact the CTEM technical staff to select the most appropriate grid type and staining conditions for each study.

M. Others

Epoxy Resin Embedding

Epoxy resin embedding is a standard sample-preparation technique used to preserve biological and material specimens for semithin and ultrathin section...

Lab-specific experience

CTEM Expertise CTEM has extensive experience in the fixation, dehydration, infiltration and epoxy resin embedding of plant, animal, microbial and material samples. The service provides support in: Selection of the most appropriate embedding protocol Optimization of fixation and dehydration conditions Resin infiltration and sample orientation Polymerization and preparation of resin blocks Semithin and ultrathin sectioning Preparation of samples for light microscopy and TEM Troubleshooting of difficult or poorly infiltrated samples Users are encouraged to contact the CTEM technical staff before sample collection or fixation to determine the most appropriate preparation and embedding protocol for their study.

M. Others

Acrylic Resin Embedding – LR Gold

LR Gold is a low-viscosity acrylic resin designed for the low-temperature embedding of biological samples. It is particularly suitable for studies in...

Lab-specific experience

CTEM Expertise CTEM provides support for the preparation and embedding of biological samples in LR Gold acrylic resin for light and transmission electron microscopy. The service may include: Advice on sample collection and fixation Selection of low-temperature processing conditions Dehydration and resin infiltration Orientation and embedding of samples Light polymerization of LR Gold Semithin sectioning for light microscopy Ultrathin sectioning for TEM Preparation of sections for immunolabelling Post-embedding immunogold techniques Optimization of protocols for fixation-sensitive antigens Users should contact the CTEM technical staff before collecting or fixing samples. Early consultation is particularly important when immunolabelling is planned, as fixation, dehydration, resin polymerization and sectioning conditions must be adapted to the target antigen and antibody.

Brightfield Microscopy (BFM)

Paraffin Wax Embedding

Paraffin wax embedding is a widely used histological technique for preparing thin sections of biological tissues for light microscopy. The method pro...

Lab-specific experience

CTEM Expertise CTEM provides support for the preparation and paraffin embedding of biological samples for light-microscopy applications. The service may include: Advice on sample collection and fixation Sample trimming and orientation Tissue dehydration and clearing Paraffin infiltration and embedding Preparation of paraffin blocks Microtome sectioning Collection of sections on glass slides Preparation of serial sections Optimization of section thickness and cutting conditions Preparation of sections for histological, immunohistochemical and image-analysis studies Users should contact the CTEM technical staff before sample collection or fixation to determine the most appropriate processing, embedding and sectioning protocol for their study.

M. Others

Immunolocalization by Light and Electron Microscopy

Immunolocalization techniques use antibodies to detect and determine the spatial distribution of specific proteins, antigens or other molecular target...

Lab-specific experience

CTEM Expertise CTEM provides support for immunolocalization studies using light, fluorescence, confocal and transmission electron microscopy. The service may include: Advice on experimental design and antibody selection Optimization of fixation and sample-preparation conditions Preparation of paraffin, cryostat or resin sections Immunofluorescence and chromogenic immunolabelling Single and multiple antigen detection Preparation of samples for confocal microscopy Pre-embedding and post-embedding immunolabelling Immunogold labelling of ultrathin sections Preparation of LR Gold sections for immuno-TEM Selection of appropriate electron-microscopy grids TEM acquisition of immunogold-labelled samples Qualitative and quantitative analysis of immunolabelling Evaluation of labelling specificity and background Users should contact the CTEM technical staff before sample collection or fixation. Early consultation is essential because fixation, embedding, sectioning and labelling conditions must be adapted to the antigen, antibody and required level of spatial resolution.

- FLIM- Fluorescence Lifetime Imaging Microscopy

Fluorescence Lifetime Imaging Microscopy (FLIM)

Fluorescence Lifetime Imaging Microscopy (FLIM) is an advanced imaging technique that measures the average time a fluorophore remains in its excited s...

Förster Resonance Energy Transfer (FRET)

FRET- Förster Resonance Energy Transfer

Förster Resonance Energy Transfer (FRET) is a fluorescence-based technique used to study molecular interactions, conformational changes and spatial pr...

Brightfield Microscopy (BFM)

Brightfield Microscopy

Brightfield microscopy is one of the most widely used optical microscopy techniques for the observation of biological and material samples. It produce...

Transmission Electron Microscopy (TEM)

TEM

Transmission Electron Microscopy (TEM) is a high-resolution imaging technique used to examine the internal ultrastructure of biological and material s...

Laser Scanning Confocal Microscopy (LSCM)

Leica Stellaris Platform

Laser Scanning Confocal Microscopy (LSCM) is an advanced fluorescence imaging technique that provides high-resolution optical sections of biological s...

- Live Cell Imaging

Live-Cell Imaging & Time-Lapse fluorescence microscopy

The Leica STELLARIS platform can be used for live-cell imaging and time-lapse fluorescence microscopy, allowing biological processes to be monitored i...

Fluorescence Recovery After Photobleaching (FRAP)

Photobleaching Techniques

Fluorescence Recovery After Photobleaching (FRAP) is a quantitative fluorescence microscopy technique used to investigate the mobility, diffusion, exc...

Fluorescence Loss in Photobleaching (FLIP)

FLIP – Fluorescence Loss in Photobleaching

Fluorescence Loss in Photobleaching (FLIP) is a fluorescence microscopy technique used to investigate molecular mobility, connectivity and exchange be...

Laser Scanning Confocal Microscopy (LSCM)

Spectral imaging and spectral unmixing

Spectral imaging is an advanced fluorescence microscopy technique that records the spectral characteristics of the fluorescence emitted by a biologica...

Laser Scanning Confocal Microscopy (LSCM)

Photoactivation, Photoconversion and Photoswitching

Photoactivation, photoconversion and photoswitching are fluorescence microscopy techniques used to selectively alter the fluorescent state of light-re...

- Photobleaching Techniques/ iFRAP-Inverse Fluorescence Recovery After...

iFRAP – Inverse FRAP

Inverse Fluorescence Recovery After Photobleaching (iFRAP) is a quantitative fluorescence microscopy technique used to investigate the retention, rele...

Laser Scanning Confocal Microscopy (LSCM)

FLAP – Fluorescence Localization After Photobleaching

Fluorescence Localization After Photobleaching (FLAP) is a ratiometric fluorescence microscopy technique used for the localized photolabelling and sub...

Laser Scanning Confocal Microscopy (LSCM)

N&B - Number and Brightness Analysis

Number and Brightness analysis (N&B) is a fluorescence fluctuation technique used to investigate the concentration, molecular brightness, aggregat...

Laser Scanning Confocal Microscopy (LSCM)

Quantitative colocalization analysis

Quantitative colocalization analysis is an image-analysis approach used to determine the degree of spatial association between two or more fluorescent...

Laser Scanning Confocal Microscopy (LSCM)

Fluorescent biosensor imaging

Fluorescent biosensor imaging is an advanced microscopy approach used to visualize and quantify biochemical activities, molecular signals and physiolo...

Laser Scanning Confocal Microscopy (LSCM)

LIGHTNING Confocal Super-Resolution Imaging

LIGHTNING is a confocal super-resolution method developed by Leica Microsystems to improve the spatial resolution, contrast and structural detail of f...

Laser Scanning Confocal Microscopy (LSCM)

Multicolour and Three-Dimensional Imaging

Multicolour fluorescence imaging enables several molecular targets, cellular compartments or biological structures to be visualized within the same sa...

Laser Scanning Confocal Microscopy (LSCM)

Time-Lapse and Four-Dimensional (4D) Imaging

Time-lapse fluorescence imaging is used to monitor biological processes by acquiring a sequence of images from the same specimen at defined time inter...

Training Activities

Preparation and Processing of Plant, Microbial and Animal Samples, and...

Hands-on/Practical Session M. Others Stereomicroscopy Transmission Electron Microscopy (TEM) Brightfield Microscopy (BFM) Laser Scanning Confocal Microscopy (LSCM) Widefield Fluorescence Microscopy (WFF)
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Vibratome and Cryostat Sectioning

Hands-on/Practical Session M. Others Widefield Fluorescence Microscopy (WFF)
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Grid Staining and Contrast Enhancement

Hands-on/Practical Session Transmission Electron Microscopy (TEM)
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Immunolocalization for Light and Electron Microscopy

Hands-on/Practical Session Transmission Electron Microscopy (TEM) Brightfield Microscopy (BFM)
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Transmission Electron Microscope Operation

Hands-on/Practical Session Transmission Electron Microscopy (TEM)
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Technical Resources

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