Total Internal Reflection Fluorescence (TIRF, widefield variant) Advanced Cellular Analyses Unit Salamanca

Total Internal Reflection Fluorescence (TIRF, widefield variant) at Advanced Cellular Analyses Unit

Owner Laboratory

Consejo Superior de Investigaciones Cientificas (CSIC)

Universidad de Salamanca

Salamanca

Centro de Investigación del Cáncer Campus Miguel de Unamuno 37007 Salamanca

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Utilizes an evanescent field (100–200 nm depth) to selectively excite fluorophores near the coverglass, drastically reducing background noise.