FRET- Förster Resonance Energy Transfer
About this Technology
Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Resonance energy transfer is a distance-dependent phenomenon due to the dipole-dipole interaction between the donor and acceptormolecules.
The efficiency of the energy transfer is sensitive to the molecular distances andnegligible at distances longer than 10nm for the common fluorophores used in biologicalapplications. For this reason, when FRET is combined with fluorescence microscopy in biologicalsamples, it can be used to report molecular associations and biochemical reactions allowing themto be located precisely at the subcellular level where these processes are taking place [2].
Labs Using this Technology
Advanced Digital Microscopy Core Facility (IRB Barcelona)
IRB Barcelona (Institute for Research in Biomedicine) · Barcelona
Advanced Optical Microscopy
Universitat de Barcelona · Barcelona
Advanced Optical Microscopy Service - Nanoimaging Unit
Centro Andaluz de Nanomedicina y Biotechnología (BIONAND) · Málaga
Microscopia Confocal SIdI-UAM
Universidad Autónoma de Madrid (UAM) · Madrid
Microscopy Core Facility
Universidad de Murcia · Murcia
Microscopy and Dynamic Imaging Unit
Instituto de Salud Carlos III · Madrid
Plataforma Cientifico-Tecnica de Microscopia Confocal
Instituto de Investigacion Biomedica Sant Pau · Barcelona
Servei de Microscopia
Universitat Autonoma de Barcelona · Barcelona
Servicio de Microscopia
Consejo Superior de Investigaciones Cientificas (CSIC) · Valencia
Servicio de Microscopia
INSTITUTO DE PARASITOLOGIA Y BIOMEDICINA LOPEZ NEYRA · Granada
Servicio de Microscopia Confocal y Citometria de Flujo
cicCartuja-Centro de Investigaciones Cientificas Isla de la Cartuja (CSIC-US) · Sevilla
Servicio de Microscopia Optica Avanzada IBBTEC
Consejo Superior de Investigaciones Cientificas (CSIC) · Santander
Servicio de Microscopia Optica y Confocal
Consejo Superior de Investigaciones Cientificas (CSIC) · Madrid
Servicio de Tecnicas Aplicadas a las Biociencias
Universidad de Extremadura · Badajoz
UNIDAD DE MICROSCOPIA OPTICA AVANZADA
Universidad de La Laguna · Santa Cruz de Tenerife
Unidad de Microscopia IDIVAL
IDIVAL Instituto de Investigacion Marqués de Valdecilla · Santander
Unidad de Microscopia e Imagen Molecular
FPS-Fundacion Progreso y Salud · Granada