Description
Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Resonance energy transfer is a distance-dependent phenomenon due to the dipole-dipole interaction between the donor and acceptormolecules.
The efficiency of the energy transfer is sensitive to the molecular distances andnegligible at distances longer than 10nm for the common fluorophores used in biologicalapplications. For this reason, when FRET is combined with fluorescence microscopy in biologicalsamples, it can be used to report molecular associations and biochemical reactions allowing themto be located precisely at the subcellular level where these processes are taking place [2].
Labs Using this Technology
Servicio de Microscopia Optica y Confocal
FRET- Förster Resonance Energy Transfer
Unidad de Microscopia IDIVAL
FRET- Förster Resonance Energy Transfer
Servicio de Microscopia Optica Avanzada IBBTEC
FRET- Förster Resonance Energy Transfer
Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor). Resonan...
Microscopia Confocal SIdI-UAM
FRET- Förster Resonance Energy Transfer
Estudio de interacciones entre proteínas mediante la técnica FRET (Fluorescence Resonance Energy Transfer). Modos: Acceptor Photobleaching y Sensiti...
Servicio de Tecnicas Aplicadas a las Biociencias
FRET- Förster Resonance Energy Transfer
Advanced Optical Microscopy Service - Nanoimaging Unit
FRET- Förster Resonance Energy Transfer
Advanced Optical Microscopy
FRET- Förster Resonance Energy Transfer
Servei de Microscopia
FRET- Förster Resonance Energy Transfer
Servicio de Microscopia
FRET- Förster Resonance Energy Transfer
We use FRET via acceptor photobleaching mode to study the interación between two molecules.
Servicio de Microscopia Confocal y Citometria de Flujo
FRET- Förster Resonance Energy Transfer
Unidad de Microscopia e Imagen Molecular
FRET- Förster Resonance Energy Transfer
Microscopy and Dynamic Imaging Unit
FRET- Förster Resonance Energy Transfer
UNIDAD DE MICROSCOPIA OPTICA AVANZADA
FRET- Förster Resonance Energy Transfer
Plataforma Cientifico-Tecnica de Microscopia Confocal
FRET- Förster Resonance Energy Transfer
Servicio de Microscopia
FRET- Förster Resonance Energy Transfer
Microscopy Core Facility
FRET- Förster Resonance Energy Transfer
Equipment required: Laser Scanning Confocal Microscope - FRET Sensitized Emission - FRET Acceptor PhotoBleaching
Advanced Digital Microscopy Core Facility (IRB Barcelona)
FRET- Förster Resonance Energy Transfer