Description
Leica SP5 Multispectral HyD HCS-A Confocal - Inverted Leica DMI 6000 fully motorised microscope. - 2 x HyD Hybrid GaASP Detectors (significantly better sensitivity and signal-noise ratio than conventional PMTs)
- 3 x PMT
- 1 transmitted light detector.
- Tandem Scanner – HQ conventional scanner and high-speed resonant scanner - AOBS (Acousto-Optical beam splitter)
- Microscope enclosure with temperature, humidity and CO2 control.
- High precision Super Z motorized stage with multi-point and multi-tile software control. 3nm resolution galvometric z-axis control
- Leica HCS-A "Matrix" software for High Content Screening applications
Objectives
- Plan Apo Oil immersion: 100x (1.4 NA), 63x (1.4 NA Blue), 40x (1.3 NA) o Plan Apo 20x (0.7 NA)
- Multi-immersion (water, glycerol, oil) 0.26mm WD
- Plan Apo 10x (0.4 NA) dry.
Laser lines (9 in the visible range)
Violet 405nm (50mW), Blue Argon 458nm, 476nm, 488nm, 496nm, 514nm (65mW) Green
DPSS 561nm (20mW), Orange HeNe 594nm (2mW), Red HeNe 633nm.
Additional Details
external academic users and companies. Please contact Dr. John Pearson (+34
952 367 634 / jrpearson@bionand.es) for more information.
Applicable Technologies
- Brightfield & other Transmission Microscopy Contrasts
Brightfield & other Transmission Microscopy Contrasts
Brightfield illumination has been one of the most widely used observation modes in optical microscopy for the past 300 years. The technique is best su...
- FRET- Förster Resonance Energy Transfer
FRET- Förster Resonance Energy Transfer
Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Reso...
- HCS- High-Content Screening
HCS- High-Content Screening
High-Content Screening (HCS) is a powerful technology which gathers Biology and Engineeringaround hardware development and experimental protocols opti...
- High Speed Laser Scanning Confocal Systems
High Speed Laser Scanning Confocal Systems
none
- Laser Scanning Confocal Microscopy
Laser Scanning Confocal Microscopy
Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimina...
- Live Cell Imaging
Live Cell Imaging
Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...
- Photobleaching Techniques/ FLIP-Fluorescence Loss In Photobleaching
Photobleaching Techniques/ FLIP-Fluorescence Loss In Photobl...
This technique is used to study the diffusion of molecules among different connected/communicated cell compartments. A fluorescent cell is repeatedly...
- Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photoble...
Photobleaching Techniques/ FRAP-Fluorescence Recovery After...
Photobleaching techniques are used as a strategy to reveal the diffusion/binding dynamics of molecules or the exchange between compartments in live ce...
- Photoconversion
Photoconversion
Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...