Description
Inverted Nikon microscope for laser-based TIRF and N-STORM super-resolution microscopy
- Motorised TIRF mirror
- Multipass TIRF dichroic filter (allows high-speed multicolour TIRF)
- Andor iXon3 897 EM-CCD camera
- Microscope enclosure with temperature, humidity and CO2 control
- High precision motorized stage
- Nikon PFS (Perfect Focus System) for improved stability
N-STORM Super-resolution
- Multipass N-Storm filter allowing fast excitation of Reporter (Alexa647) and multiple Activator (e.g. Alexa405, Cy2 and Cy3) fluorochromes.
- Integrated acquisition and analysis modules capable of multicolor super-resolution imaging up to 20nm lateral resolution.
- dSTORM compatible
- Cylindrical lens allowing 3D super-resolution point mapping up to 60nm axially.
- Additional workstation with N-STORM module for offline image processing
Objective lenses:
o Plan Apo Oil immersion: 100x (1.49 NA) TIRF o Plan Fluor 40x (0.75 NA)
o Plan Apo 10x (0.45 NA).
Lasers
- Violet 405nm
- Argon-Ion 457nm, 477nm, 488nm, 514nm
- Yellow Diode 561nm
- High power Red 647nm (300mW) for N-STORM
Additional Details
external academic users and companies. Please contact Dr. John Pearson (+34
952 367 634 / jrpearson@bionand.es) for more information.
Applicable Technologies
- Brightfield & other Transmission Microscopy Contrasts
Brightfield & other Transmission Microscopy Contrasts
Brightfield illumination has been one of the most widely used observation modes in optical microscopy for the past 300 years. The technique is best su...
- Live Cell Imaging
Live Cell Imaging
Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...
- Super Resolution/ Stochastic Optical Reconstruction microscopy (STOR...
Super Resolution/ Stochastic Optical Reconstruction microsco...
Stochastic Optical Reconstruction Microscopy (STORM), as well as PALM or fPALM, is a super-resolution imaging technique that utilizes sequential activ...
- Total Internal Reflection Fluorescence Microscopy (TIRF)
Total Internal Reflection Fluorescence Microscopy (TIRF)
Critical angle incidence of a laser beam onto the inner surface of a coverglass creates an evanescent field which illuminates the field of view with a...
- Wide Field Fluorescence Microscopy
Wide Field Fluorescence Microscopy
Conventional fluorescence excitation, upright or inverted, incl. Hg lamp, Metal-Halide or LED illumination.