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FLIM- Fluorescence Lifetime Imaging Microscopy

Fluorescence lifetime imaging microscopy (FLIM) — single-photon or multiphoton Servicio de Microscopia Optica Avanzada del Centro Nacional de Biotecnologia Madrid
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Equipment
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Technical Resources

Description

Measures the fluorescence decay rate, providing information on the molecular microenvironment (pH, Ca2+, quenching) independent of intensity.

How this lab uses this technology

Measures the fluorescence decay rate, providing information on the molecular microenvironment (pH, Ca2+, quenching) independent of intensity.

Equipment

Leica Microsystems Stellaris 8 STED 3X FALCON DLS / Leica Microsystems...

Stellaris 8

Leica Microsystems Stellaris 8 STED 3X FALCON DLS Stimulated emission depletion (STED) microscopy