Description
White laser with excitation range 440 to 790 nm and 405 nm diode.
Two Power HyD S spectral detectors and two Power HyD X spectral detectors.
STED 3X nanoscopy module with three depletion lines: 592, 660 and 775 nm.
FLIM (Fluorecence Lifetime Imaging Microscopy) system.
FCS (Fluorecence Correlation Spectroscopy) system.
DLS (Digital Light Sheet) mesoscopy system.
Integrated software module for real-time multidimensional super-resolution multidimensional image detection and processing (Lightning).
Environmental control camera (Okolab) for time-lapse experiments.
Additional Details
Applicable Technologies
Laser Scanning Confocal Microscopy (LSCM)
Laser Scanning Confocal Microscopy
Employs a pinhole aperture and focused laser beams to collect serial optical sections, allowing for high-contrast 3D reconstruction.
Fluorescence Recovery After Photobleaching (FRAP)
Photobleaching Techniques/ FRAP-Fluorescence Recovery After...
Photobleaching techniques used to measure the mobility, diffusion coefficients, and exchange rates of tagged molecules.
Förster Resonance Energy Transfer (FRET)
FRET- Förster Resonance Energy Transfer
Distance-dependent energy transfer used as a molecular ruler (1–10 nm) to monitor protein interactions and conformational changes.
Fluorescence Correlation Spectroscopy (FCS)
FCS- Fluorescence Correlation Spectroscopy
Analyzes intensity fluctuations in a tiny volume to determine molecular concentration, diffusion, and binding kinetics.
Fluorescence lifetime imaging microscopy (FLIM) — single-photon or mul...
FLIM- Fluorescence Lifetime Imaging Microscopy
Measures the fluorescence decay rate, providing information on the molecular microenvironment (pH, Ca2+, quenching) independent of intensity.
Stimulated Emission Depletion (STED)
Super Resolution/ Stimulated Emission Depletion microscopy (...
Uses a doughnut-shaped depletion laser to quench peripheral fluorescence, effectively narrowing the focal spot to sub-50 nm levels.