Advanced Optical Microscopy

Bioluminescence Imaging (BLI)

Bioluminescence imaging (BLI) is a technology that allows for the noninvasive study of ongoing biological processes in small laboratory animals. Biolu...

Brightfield & other Transmission Microscopy Contrasts

Brightfield illumination has been one of the most widely used observation modes in optical microscopy for the past 300 years. The technique is best su...

FRET- Förster Resonance Energy Transfer

Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Reso...

High Speed Laser Scanning Confocal Systems

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Image Analysis

Image Analysis/ Colocalization

In vivo Imaging (animal)

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Labelling/ Fluorescent Proteins

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Labelling/ Fluorophores

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Labelling/ Fluorophores/ Spectra

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Laser Scanning Confocal Microscopy

Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimina...

Live Cell Imaging

Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...

Microinjection

Microinjection is a method for injecting substances into a single living cell using a glass micropipette under a microscope. It is a simple mechanical...

Non-Linear Imaging/ Multiphoton fluorescence Imaging

Multiphoton microscopy uses pulsed long-wavelength light to excite fluorophores within the specimen being observed. The fluorophore absorbs the energy...

Non-Linear Imaging/ SHG-Second Harmonic Generation

In biological and medical science, the effect of second harmonic generation is used for high-resolution optical microscopy. Because of the non-zero se...

Photoactivation PA-FPs

Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...

Photobleaching Techniques/ FLIP-Fluorescence Loss In Photobl...

This technique is used to study the diffusion of molecules among different connected/communicated cell compartments. A fluorescent cell is repeatedly...

Photobleaching Techniques/ FRAP-Fluorescence Recovery After...

Photobleaching techniques are used as a strategy to reveal the diffusion/binding dynamics of molecules or the exchange between compartments in live ce...

Photobleaching Techniques/ iFRAP-Inverse Fluorescence Recove...

It is a variant of photobleaching techniques FRAP where all the fluorescent molecules in a cell except for a small region are bleached. The loss of fl...

Photoconversion

Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...

Wide Field Fluorescence Microscopy

Conventional fluorescence excitation, upright or inverted, incl. Hg lamp, Metal-Halide or LED illumination.