- Brightfield & other Transmission Microscopy Contrasts

Brightfield & other Transmission Microscopy Contrasts

Brightfield illumination has been one of the most widely used observation modes in optical microscopy for the past 300 years. The technique is best su...

- FRET- Förster Resonance Energy Transfer

FRET- Förster Resonance Energy Transfer

Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Reso...

- High Speed Laser Scanning Confocal Systems

High Speed Laser Scanning Confocal Systems

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- In vivo Imaging (animal)

In vivo Imaging (animal)

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- Laser Scanning Confocal Microscopy

Laser Scanning Confocal Microscopy

Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimina...

- Live Cell Imaging

Live Cell Imaging

Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...

- Non-Linear Imaging/ Multiphoton fluorescence Imaging

Non-Linear Imaging/ Multiphoton fluorescence Imaging

Multiphoton microscopy uses pulsed long-wavelength light to excite fluorophores within the specimen being observed. The fluorophore absorbs the energy...

- Non-Linear Imaging/ SHG-Second Harmonic Generation

Non-Linear Imaging/ SHG-Second Harmonic Generation

In biological and medical science, the effect of second harmonic generation is used for high-resolution optical microscopy. Because of the non-zero se...

- Photoactivation PA-FPs

Photoactivation PA-FPs

Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...

- Photobleaching Techniques/ FLIP-Fluorescence Loss In Photobleaching

Photobleaching Techniques/ FLIP-Fluorescence Loss In Photobl...

This technique is used to study the diffusion of molecules among different connected/communicated cell compartments. A fluorescent cell is repeatedly...

- Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photoble...

Photobleaching Techniques/ FRAP-Fluorescence Recovery After...

Photobleaching techniques are used as a strategy to reveal the diffusion/binding dynamics of molecules or the exchange between compartments in live ce...

- Photobleaching Techniques/ iFRAP-Inverse Fluorescence Recovery After...

Photobleaching Techniques/ iFRAP-Inverse Fluorescence Recove...

It is a variant of photobleaching techniques FRAP where all the fluorescent molecules in a cell except for a small region are bleached. The loss of fl...

- Photoconversion

Photoconversion