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Two photon microscopy (2P)

D. Nonlinear / Multiphoton Microscopy Achucarro Basque Center for Neurosciences - Imaging Facility Leioa
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Description

Imaging based on non-linear light-matter interactions, often requiring high-intensity pulsed lasers.

How this lab uses this technology

Two-photon microscopy enables fluorescence imaging of living biological samples with increased penetration depth and reduced photodamage, making it well suited for functional and structural studies in thick tissues. It supports applications such as live morphology visualization, calcium imaging, and electrophysiology-coupled measurements, in both in vitro and in vivo configurations

Equipment