Total Internal Reflection Fluorescence (TIRF, widefield variant)
BRALM
Bilbao / Leioa
Total Internal Reflection Fluorescence (TIRF, widefield variant) at Basque Resource for Advanced Light Microscopy
About the Installation
Utilizes an evanescent field (100–200 nm depth) to selectively excite fluorophores near the coverglass, drastically reducing background noise.