Technology
Total Internal Reflection Fluorescence Microscopy (TIRF)
About this Technology
Critical angle incidence of a laser beam onto the inner surface of a coverglass creates an evanescent field which illuminates the field of view with a typical depth of 100 to 200nm. Technique dedicated to probes sitting at the glass surface, and performing only at high NA, typically beyond 1.45.
Labs Using this Technology
ADM
Advanced Digital Microscopy Core Facility (IRB Barcelona)
IRB Barcelona (Institute for Research in Biomedicine) · Barcelona
ALMU
Advanced Light Microscopy Unit
Centre for Genomic Regulation · Barcelona
BION
Advanced Optical Microscopy Service - Nanoimaging Unit
Centro Andaluz de Nanomedicina y Biotechnología (BIONAND) · Málaga
AFM/
Laboratorio de AFM/Fluorescencia
IMDEA Nanociencia · Madrid
MICR
Microscopy and Dynamic Imaging Unit
Instituto de Salud Carlos III · Madrid
STAB
Servicio de Tecnicas Aplicadas a las Biociencias
Universidad de Extremadura · Badajoz
MICR
Unidad de Microscopia IDIVAL
IDIVAL Instituto de Investigacion Marqués de Valdecilla · Santander