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Microscopia Confocal SIdI-UAM

Microscopia Confocal

Universidad Autónoma de Madrid (UAM)
Servicio Interdepartamental de Investigación (SIdI) UAM
Madrid
3
Equipment
8
Technologies
0
Training

Equipment

Available Technologies

- Image Analysis

Image Analysis

Tipo ensayo 1. PROCESAMIENTO • Optimización de la imagen • Edición de imagen: cortar, pegar, cambiar profundidad bits, alinear imágenes, combinar y...

Lab-specific experience

• Image J para microscopía. V Jornadas de Formación. 10 y 11 de Febrero de 2011. Centros Científicos y Tecnológicos, Universidad de Barcelona. • III Curso Práctico de Microscopía Optimizada por Ordenador. 20-23 de Junio de 2011. Centros Científicos y Tecnológicos, Universidad de Barcelona. • Photoshop. Diseño gráfico. 10-24 Mayo 2006. CAM.

- FRET- Förster Resonance Energy Transfer

FRET- Förster Resonance Energy Transfer

Estudio de interacciones entre proteínas mediante la técnica FRET (Fluorescence Resonance Energy Transfer). Modos: Acceptor Photobleaching y Sensitize...

Lab-specific experience

Oncogene. 2005 Jun 16;24(26):4206-19. The adaptor Grb7 is a novel calmodulin-binding protein: functional implications of the interaction of calmodulin with Grb7.*** Li H , Sánchez-Torres J , del Carpio AF , Nogales-González A , Molina-Ortiz P , Moreno MJ , Török K , Villalobo A .

- Labelling/ Fluorophores

Labelling/ Fluorophores

Anticuerpos secundarios fluorescentes, medios de montaje y otros reactivos en stock (Life Technologies, Jackson Inmunoresearch, Ibidi, Electron Micro...

- Laser Scanning Confocal Microscopy

Laser Scanning Confocal Microscopy

Leica TCS SP2 Leica TCS SP5

Lab-specific experience

AÑO 2012 PLoS One. 2012;7(9):e45260. doi: 10.1371/journal.pone.0045260. Epub 2012 Sep 18. NOD1 Activation Induces Cardiac Dysfunction and Modulates Cardiac Fibrosis and Cardiomyocyte Apoptosis. Fernández-Velasco M , Prieto P , Terrón V , Benito G , Flores JM , Delgado C , Zaragoza C , Lavin B , Gómez-Parrizas M , López-Collazo E , Martín-Sanz P , Boscá L . http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0045260 Front. Pharmacol., 05 June 2012 | doi: 10.3389/fphar.2012.00103 Anticontractile effect of perivascular adipose tissue and leptin are reduced in hypertension Beatriz Gálvez-Prieto, Beatriz Somoza , Marta Gil-Ortega , Concha F. García-Prieto, Ana I. de las Heras, M. Carmen González , Silvia Arribas, Isabel Aranguez, Juliane Bolbrinker, Reinhold Kreutz, Mariano Ruiz-Gayo and Maria S. Fernández-Alfonso http://www.frontiersin.org/Cardiovascular_and_Smooth_Muscle_Pharmacology/10.3389/fphar.2012.00103/full PlosOne, January 2012: Volume 7, Issue 1. AKT Signaling Mediates IGF-I Survival Actions on Otic Neural Progenitors. Maria R. Aburto, Marta Magariños, Yolanda Leon, Isabel Varela-Nieto, Hortensia Sanchez-Calderon. http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0030790 AÑO 2010 Endocrinology. 2010 Jul;151(7):3299-306. Epub 2010 Apr 21. Adaptative nitric oxide overproduction in perivascular adipose tissue during early diet-induced obesity. Gil-Ortega M , Stucchi P , Guzmán-Ruiz R , Cano V , Arribas S , González MC , Ruiz-Gayo M , Fernández-Alfonso MS , Somoza B . http://endo.endojournals.org/content/151/7/3299.long Reproduction. 2010 Mar;139(3):523-32. Epub 2009 Dec 23. Influence of leptin on in vitro maturation and steroidogenic secretion of cumulus-oocyte complexes through JAK2/STAT3 and MEK 1/2 pathways in the rabbit model. Arias-Alvarez M , García-García RM , Torres-Rovira L , González-Bulnes A , Rebollar PG , Lorenzo PL . http://www.ncbi.nlm.nih.gov/pubmed/20032210?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=1 Kidney Int. 2010 Feb 17. [Epub ahead of print] Differential resolution of inflammation and recovery after renal ischemia-reperfusion injury in Brown Norway compared with Sprague Dawley rats. Sáenz-Morales D , Conde E , Blanco-Sánchez I , Ponte B , Aguado-Fraile E , de Las Casas G , García-Martos M , Alegre L , Escribese MM , Molina A , Santiuste C , Liaño F , García-Bermejo ML . http://www.ncbi.nlm.nih.gov/pubmed/20164827?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=1 Autophagy. 2010 Jan;6(1):100-9. Epub 2010 Jan 18. Autophagy dysfunction and ubiquitin-positive protein aggregates in Dictyostelium cells lacking Vmp1. Calvo-Garrido J , Escalante R . http://www.ncbi.nlm.nih.gov/pubmed/20009561?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=1 Theriogenology. 2010 Jan;73(1):26-35. Epub 2009 Oct 27. Influence of hormonal and nonhormonal estrus synchronization methods on follicular and oocyte quality in primiparous lactating does at early postpartum period. Arias-Alvarez M , García-García RM , Torres-Rovira L , González-Bulnes A , Rebollar PG , Lorenzo PL . http://www.ncbi.nlm.nih.gov/pubmed/19864013?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=3 Experimental Physiology 00.00 pp 1–11 Enhanced survival of vascular smooth muscle cells accounts for heightened elastin deposition in arteries of neonatal spontaneously hypertensive rats Silvia M. Arribas, Carmen Hermida, M. Carmen González, Yanting Wang and Aleksander Hinek.

- Live Cell Imaging

Live Cell Imaging

Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...

- Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photoble...

Photobleaching Techniques/ FRAP-Fluorescence Recovery After...

Photobleaching techniques are used as a strategy to reveal the diffusion/binding dynamics of molecules or the exchange between compartments in live ce...

- In vivo Imaging (animal)

In vivo Imaging (animal)

none

- Image Analysis/ Colocalization

Image Analysis/ Colocalization