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Microscopy Core Facility

SUM

Universidad de Murcia
Área Científica y Técnica de Investigación (ACTI)
Murcia
4
Equipment
5
Technologies
0
Training

Equipment

Leica Microsystems Leica TCS SP8 / Leica Leica TCS SP8

Leica Microsystems Leica TCS SP8 Laser Scanning Confocal Microscope

Leica Microsystems Laser Scanning Confocal TCS-SP2 / Leica Laser Scann...

Leica Microsystems Laser Scanning Confocal TCS-SP2 Laser Scanning Confocal Microscope

Nikon Eclipse 90i / Nikon Eclipse 90i

Nikon Eclipse 90i Laser Scanning Confocal Microscope

Leica Microsystems DMR / Leica DMR

Leica Microsystems DMR

Available Technologies

- Labelling/ Fluorophores

Labelling/ Fluorophores

- Equipment required: Laser Scanning Confocal Microscopy - Applications: Spatial distribution of macromolecules in fixed cells. • Direct immunofluo...

Lab-specific experience

Immunofluorescence is a technique that requires fluorescent-labeled antibodies to detect specific target antigens.

- Labelling/ Fluorescent Proteins

Labelling/ Fluorescent Proteins

Equipment required: Laser Scanning Confocal Microscopy - Applications: Spatial distribution movement and interactions of macromolecules in living ce...

Lab-specific experience

Live cell dyes, fluorescent protein tags and other methods to fluorescently label proteins of interest give us a range of tools to investigate virtually any cellular process under the confocal microscope.

- FRET- Förster Resonance Energy Transfer

FRET- Förster Resonance Energy Transfer

Equipment required: Laser Scanning Confocal Microscope - FRET Sensitized Emission - FRET Acceptor PhotoBleaching

Lab-specific experience

The FRET technique allows insight into the interactions between proteins or molecules in proximities beyond light microscopic resolution.

- Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photoble...

Photobleaching Techniques/ FRAP-Fluorescence Recovery After...

Equipment: Laser Scanning Confocal Microscope

Lab-specific experience

This technique could be used to characterize protein mobility and turnover rate.

- Live Cell Imaging

Live Cell Imaging

Live-cell imaging techniques provide critical insight into the fundamental nature of cellular and tissue function, especially due to the rapid advance...

Lab-specific experience

Vesicle trackingLine scan to measure Ca2+ sparks pH measurement Colocalization

Technical Resources

  • Inmunofluorescence optimized protocol

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