Description
Confocal Laser Scanning Microscope (CLSM) LEICA TCS SP5 fitted with nine excitation lines (405, 458, 476, 488, 496, 514, 543, 594 and 633 nm) from UV-Diode, Ar and HeNe lasers and AOTF (Acousto-Optical Tunable Filter) – AOBS (Acousto-Optical Beam Splitter) System.
Additional Details
Motorized stage allows to mark and memorize multiple positions and to perform tile-scans.
Posible to adquire simultaneusly fluorescence and transmited light. Up to 4 PMT (3 fluorescence + 1 DIC).
Suited for mulilabeling experiments, FRET and FRAT.
Applicable Technologies
- Brightfield & other Transmission Microscopy Contrasts
Brightfield & other Transmission Microscopy Contrasts
Brightfield illumination has been one of the most widely used observation modes in optical microscopy for the past 300 years. The technique is best su...
- Laser Scanning Confocal Microscopy
Laser Scanning Confocal Microscopy
Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimina...
- Image Analysis/ Software
Image Analysis/ Software
LAS AF software (Leica). Leica propietary 3D deconvolution license. FRET-SE wizard. FRET-AB wizard. FRAT wizard. Live Data Mode Tool for complex time-...
- FRET- Förster Resonance Energy Transfer
FRET- Förster Resonance Energy Transfer
Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor). Resonanc...
- Image Analysis
Image Analysis
- Live Cell Imaging
Live Cell Imaging
Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...
- Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photoble...
Photobleaching Techniques/ FRAP-Fluorescence Recovery After...
Photobleaching techniques are used as a strategy to reveal the diffusion/binding dynamics of molecules or the exchange between compartments in live ce...