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Equipment
Wide Field Fluorescence Microscope

Leica Microsystems AF6000 LX / Multidimensional

Leica Microsystems
AF6000 LX

Description

Multidimensional Microscopy System Leica AF6000 LX for in vivo cell imaging.
It is equipped with an incubation camera with CO2 and temperature controlled and motorized XY Scanning Stage. Camera CCD Hamamatsu 9100-02.

Multidimensional acquisition of images in X,Y,Z,t and ʎ. Equipped with a wide range of fluorescence filters spanning whole visible spectrum from UV to near IR. Being fully motorized in temporal acquisition.

  • FRET (Förster resonance energy transfer) studies with CFP_YFP tandem.

  • Calcium in vivo studies with FURA and other dynamic studies.

  • Motorized applications: Mark & Find enables the user to define multi position experiments to observe a large number of cells within one experiment. Is particularly useful for studying dynamic processes in live cells. And the Tile Scan feature allows the acquisition of overview images of the specimen with multiple channels and in three dimensions.

Lenses / Objectives

2.5X, 5X, 10X, 20X, 40X, 63X oil and 100X oil

Additional Details

FILTERS
CHROMA: Reflector BF, BGR, CFP/YFP, A4, L5, N3, Y5, FURA
SEMROCK: GFP y TXRed

Applicable Technologies

2