Description
Inverted Axiovert 200M motorized microscope with an EMCCD C9100-02 (Hamamatsu)
Motorized stage (Marzhauser)
High speed excitation and emission filter wheels (Sutter)
Incubation chamber for the microscope
Metamorph
Additional Details
- 10X/0.45 Plan-Apochromat Ph1 (BF, DIC II and Ph1)
- 20X/0.6 Plan-Apochromat Ph2 (BF, DIC II and Ph2)
- 40X/1.3 oil Plan-Neofluar Ph3 (Oil). (BF, DIC III and Ph3)
- 63X/1.4 oil Plan-Apochromat Ph3 (Oil) (BF, DIC III and Ph3)
- 63x/1.2 Water C-Apochromat Corr (Water) (BF and DICIII)
- 100X/1.45 oil Plan-Fluar (Oil) ((BF)
• Fluorescence filters
- Cyan FP ( 426-446/422-432, 455 DCLP, 460-500)
- Yellow FP (490-510, 510-560, HQ520LP)
- Yellow FP BP (HQ 520-550/528.5-555.5)
- Dichroic CFP/YFP (440-500 510-610)
- GFP y DsRed (S473-498 S552-577 86007bs S500-540 S590-650)
- Rodamine (Em 560/40)
- Cy5 (HQ590-650, Q660LP, HQ662.5-737.5)
- Dapi (359-371, 450LP)
• Optics
- Brightfield (BF)
- Nomarski (DIC)
- Phase contrast (Ph)
- Fluorescence
• Digital camera
- C9100-02 (Hamamatsu)
• Computer
- Display Mitsubishi 19 "
- Windows XP x32
- Metamorph 7.1 r2 (Universal Imaging)
• Fluorescence
- High speed emission and excitation filter wheels: Sutter LAMBDA 10-2
- Motorized stage (Marzhauser)
- Xenon XBO 75W/2
• Incubation system (Cell Observer-Zeiss)
- Temperature control
- CO2 level control
- Humedity control
- Microscope incubator
- Perfusion system
Applicable Technologies
- Brightfield & other Transmission Microscopy Contrasts
Widefield, Darkfield, Phase contrast (Ph), Differencial Interference Contrast (DIC),
- Live Cell Imaging
Sistema de incubación de PeCon que engloba a casi todo el microscopio con control de temperatura, humedad y pH
- FRET- Förster Resonance Energy Transfer
FRET- Förster Resonance Energy Transfer
Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Reso...
- Wide Field Fluorescence Microscopy
Wide Field Fluorescence Microscopy
Conventional fluorescence excitation, upright or inverted, incl. Hg lamp, Metal-Halide or LED illumination.