Equipment
Laser Scanning Confocal Microscope
Multiphoton Microscope
Zeiss (Carl Zeiss Microscopy Gmbh) Multiphoton / Zeiss Multiphoton With an AxioImager M1 upright and one AxioObserver inverted microscopes
With an AxioImager M1 upright and one AxioObserver inverted microscopes
Description
Confocal and Multiphoton system with two microscopes:
1) LSM710 with an AxioObserver inverted microscope.
2 fluorescence detectors + 1 Quasar spectral detector + 1 transmission detector
2 Non-descanned detectors
Laser lines: 405/458/488/514/561/633/Infrared MaiTai Deep Sea
Incubation chamber for the microscope
2) LSM510 with an upright AxioImager M1.
1 fluorescence detector + 1 transmission detector 2 Non-descanned detectors Laser lines: 543/Infrared MaiTai Deep Sea
Additional Details
• Objectives
- 5x/0,16 EC "Plan-Neofluar" M27 (Brightfield)
- 10X/0.3 EC Plan-Neofluar M27 (Brightfield)
- 25x/0,8 LCI "Plan-Neofluar" Imm Corr DIC M27 (Oil. Brightfield and DIC II)
- 20X/0.8 Plan-Apochromat (Brightfield and DIC II)
- 40x/1,30 EC "Plan-Neofluar" Oil DIC M27 (Oil. Brightfield and DIC III)
- 63X/1.4 Plan-Apochromat Oil DIC M27 (Oil. Brightfield and DIC III)
- 100x/1,40 Plan-Apochromat" Oil DIC M27 (Oil. Brightfield and DIC III)
- 40x/1,2 Water C-Apochromat" Corr UV-VIS-IR M27 (Water and immersion oil with RI 1.3. Brightfield and DIC III)
- 63x/1.2 Water C-Apochromat Corr UV-VIS-IR M27 (Water and immersion oil with RI 1.3. Brightfield and DIC III)
- 20x/0.5 Water N-Achroplan (Dipping. Brightfield and DIC II)
- 40x/1.0 Water Plan-Apochromat (Dipping. Brightfield and DIC III)
- 63x/1.0 Water Plan-Apochromat (Dipping. Brightfield and DIC III)
• Fluorescence filters
- DAPI/GFP (Ex 395-420/475-500 Em 445-470/510-570) (51000 Chroma)
- CFP/DsRed (Ex 400-440/525-580 Em 460-510/590-710) (55018 Chroma)
• Laser
- Diode 405 nm (on inverted microscope)
- Argon 458/488/514 nm (on inverted microscope)
- HeNe 543nm (on upright microscope)
- DPSS 561 nm (on inverted microscope)
- HeNe 633nm (on inverted microscope)
- MaiTai Deep Sea (Spectra Physics): 690-1020 nm, pulsos inferiores a 100 femtoseg, 2.5 Watt de potencia, 80 Mhz (on both microscopes)
• Confocal filters
“Descanned” detection:
- Inverted microscope with spectral detector: Quasar + 2 PMTs
- Emission wavelenghts: 370-760 nm
- Upright microscope with these filters: BP 390-465, BP 435-485, BP 480-520, BP 500-550, BP 560-615, BP 650-710, KP 660
“Non-Descanned” detection:
- Inverted microscope: BP 380-430, BP 455-500, BP 465-515, BP 500-550, BP 520-560, BP 540-590, BP 565-610
- Upright microscope: BP 440-485, BP 455-500, BP 495-545, BP 500-550, BP 520-560, BP 570-640
• Computer
- Windows Vista in the inverted microscope and XP in the upright one
- Software: Zeiss Zen2010B sp1 in the inverted microscope and Zen 2008 in the upright one
• In Vivo incubation system (Cell Observer-Zeiss) (Inverted microscope)
- Perfusion system
- 5x/0,16 EC "Plan-Neofluar" M27 (Brightfield)
- 10X/0.3 EC Plan-Neofluar M27 (Brightfield)
- 25x/0,8 LCI "Plan-Neofluar" Imm Corr DIC M27 (Oil. Brightfield and DIC II)
- 20X/0.8 Plan-Apochromat (Brightfield and DIC II)
- 40x/1,30 EC "Plan-Neofluar" Oil DIC M27 (Oil. Brightfield and DIC III)
- 63X/1.4 Plan-Apochromat Oil DIC M27 (Oil. Brightfield and DIC III)
- 100x/1,40 Plan-Apochromat" Oil DIC M27 (Oil. Brightfield and DIC III)
- 40x/1,2 Water C-Apochromat" Corr UV-VIS-IR M27 (Water and immersion oil with RI 1.3. Brightfield and DIC III)
- 63x/1.2 Water C-Apochromat Corr UV-VIS-IR M27 (Water and immersion oil with RI 1.3. Brightfield and DIC III)
- 20x/0.5 Water N-Achroplan (Dipping. Brightfield and DIC II)
- 40x/1.0 Water Plan-Apochromat (Dipping. Brightfield and DIC III)
- 63x/1.0 Water Plan-Apochromat (Dipping. Brightfield and DIC III)
• Fluorescence filters
- DAPI/GFP (Ex 395-420/475-500 Em 445-470/510-570) (51000 Chroma)
- CFP/DsRed (Ex 400-440/525-580 Em 460-510/590-710) (55018 Chroma)
• Laser
- Diode 405 nm (on inverted microscope)
- Argon 458/488/514 nm (on inverted microscope)
- HeNe 543nm (on upright microscope)
- DPSS 561 nm (on inverted microscope)
- HeNe 633nm (on inverted microscope)
- MaiTai Deep Sea (Spectra Physics): 690-1020 nm, pulsos inferiores a 100 femtoseg, 2.5 Watt de potencia, 80 Mhz (on both microscopes)
• Confocal filters
“Descanned” detection:
- Inverted microscope with spectral detector: Quasar + 2 PMTs
- Emission wavelenghts: 370-760 nm
- Upright microscope with these filters: BP 390-465, BP 435-485, BP 480-520, BP 500-550, BP 560-615, BP 650-710, KP 660
“Non-Descanned” detection:
- Inverted microscope: BP 380-430, BP 455-500, BP 465-515, BP 500-550, BP 520-560, BP 540-590, BP 565-610
- Upright microscope: BP 440-485, BP 455-500, BP 495-545, BP 500-550, BP 520-560, BP 570-640
• Computer
- Windows Vista in the inverted microscope and XP in the upright one
- Software: Zeiss Zen2010B sp1 in the inverted microscope and Zen 2008 in the upright one
• In Vivo incubation system (Cell Observer-Zeiss) (Inverted microscope)
- Perfusion system