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Zeiss (Carl Zeiss Microscopy Gmbh) Laser Microdissector PALM MicroBeam IV

Zeiss (Carl Zeiss Microscopy Gmbh) Laser Microdissector PALM MicroBeam IV

Description

Technical specifications

Laser microdissection system

  • ZEISS PALM MicroBeam IV mounted on a motorized ZEISS Axio Observer 7 inverted microscope.

  • UV laser-based laser microdissection with Laser Pressure Catapulting (LPC) technology for contact-free sample collection.

  • Fixed 90° laser configuration for enhanced cutting stability and precision.

Objectives

  • Fluar 5×/0.25 Air

  • Fluar 10×/0.50 Air

  • LD Plan-Neofluar 20×/0.40 Korr Air

  • LD Plan-Neofluar 40×/0.60 Korr Air

  • LD Plan-Neofluar 63×/0.75 Korr Ph2 Air

Imaging

  • Brightfield and fluorescence imaging.

  • Axiocam 305 color (5 MP CMOS) for histology and immunohistochemistry.

  • Axiocam 305 mono (5 MP CMOS) high-sensitivity monochrome camera for fluorescence imaging.

  • Simultaneous support for brightfield and fluorescence-guided laser microdissection.

Fluorescence configuration

  • Motorized fluorescence illumination (HXP 120 V).

  • Filter sets:

    • BFP (Filter Set 96 HE)

    • FITC (Filter Set 44 HE)

    • Texas Red (Filter Set 45 HQ)

    • Cy5 (Filter Set 50)

  • VIS-LED transmitted illumination.

Motorized hardware

  • Motorized RoboStage II.

  • Motorized 6-position objective turret.

  • Motorized condenser.

  • Motorized fluorescence reflector turret.

  • CapMover II automated sample collection system.

  • TubeCollector and SingleCap collectors for multiple collection formats.

Environmental control

  • On-stage incubation system.

  • Temperature-controlled stage.

  • CO₂ regulation (1–8%).

  • Humidity control.

  • Compatible with live-cell laser microdissection.

Software

  • PALM RoboSoftware 4.9 Pro.

  • LCM/AxioVision SE64 4.9.1.

  • Automated laser calibration.

  • Autofocus.

  • Image segmentation and measurement tools.

  • Time-lapse acquisition.

  • Z-stack.

  • Multichannel fluorescence.

Sample compatibility

  • Histological sections.

  • Immunohistochemistry.

  • Fluorescently labelled tissues.

  • Live-cell cultures.

  • Conventional glass slides and membrane slides (PEN/PET), including optimized workflows for membrane-free tissue sections for downstream spatial omics applications.

Applicable Technologies