Description
The Olympus IXplore SpinSR10 spinning disk confocal super resolution microscope is designed to provide super resolution (down to 120nm XY resolution) and prolonged cell viability in confocal time-lapse imaging due to less phototoxicity and bleaching.
The microscope has been adapted with a Rapp OptoElectronic UGA-42 Caliburn 355/42 pulsed laser (355nm, 1kHz, 42µJ/pulse) to perform ablation and microdissection and with a UGA‑42 Geo module for localized photomanipulation.
Lenses / Objectives
• 10x/0.40 UPLXAPO | Air | WD: 3.1mm
• 20x/0.80 UPLXAPO | Air | WD: 0.6mm
• 40x/1.30 UPLFLN | Oil | WD: 0.2mm
• 60x/1.42 UPLXAPO | Oil | WD: 0.15mm
• 100x/1.30 UPLFLN | Oil | WD: 0.2mm
• 100x/1.50 UPLAPO | Oil | WD: 0.12mm
Additional Details
Coherent OBIS Series
405 nm | Diode | 50mW
488 nm | Diode | 100mW
561 nm | Diode | 100mW
640 nm | Diode | 100mW
• Photomanipulation Laser Lines
LDI-4 Series
405 nm | Diode | 300mW
470 nm | Diode | 1W
555 nm | Diode | 1W
640 nm | Diode | 450mW
• Confocal Filters
Ex: Multi-band 405 - 488 - 561 - 640
Em: Band-pass 447/60 | 525/50 | 586/20 | 617/73 | 655/40 | 685/40
• Widefield Light Source
CoolLed pE-300ultra
• Widefield Filters
Ex: BP 352-402 | BS: 4090 | Em: LP 409
Ex: BP 460-480 | BS: 495 | Em: BP 495-540
Ex: BP 530-550 | BS: 570 | Em: LP 575
Ex: BP 565-585 | BS: 595 | Em: BP 600-690
• Camera
HAMAMATSU ORCA FusionBT
· Resolution 2304px x 2304px
· Pixel Size: 6.5µm x 6.5µm
· Effective Area: 14.976mm × 14.976mm
· Bit depth: 16bit
• Environmental control chamber (temperature, CO2, humidity) for in vivo imaging
Applicable Technologies
Laser ablation / Nano-surgery
Rapp OptoElectronic UGA-42 Caliburn 355/42 pulsed laser (355nm, 1kHz, 42µJ/pulse) to perform ablation and microdissection and with a UGA‑42 Geo module...
Spinning Disk Confocal Microscopy (SDCM)
Widefield Fluorescence Microscopy (WFF)
Differential Interference Contrast (DIC / Nomarski)
SoRa (Super-resolution Spinning Disk)
Optogenetics (light-based perturbation)