Description
Leica DMI 6000 CS inverted microscope coupled to a confocal system, with Filter A (blue), Filter I3 (green) and Filter N21 (red).
-Laser lines: Argon; 458 nm, 476 nm, 488 nm, 496 nm, 514 nm. DPSS; 561nm. HeNe; 633nm. Pulsed Lasers; 405nm, 470nm, 640nm.
-Scanners: galvano/resonant
-Objectives: HCX PL APO lambda blue 63x/1.40-0.60 Oil HCX PL APO CS 40x/1.25-0.75 Oil HC PL APO CS 20x/0.70 HC PL APO CS 10x/0.40
-Mini-Incubation chamber for CO2 and temperature control
-Acquisition software: LAS-AF 2.5.1
Applicable Technologies
- FCS- Fluorescence Correlation Spectroscopy
FCS- Fluorescence Correlation Spectroscopy
Fluorescence Correlation Spectroscopy (FCS) is a tool that provides quantitative localized measurements of important physical parameters including mec...
- FLIM- Fluorescence Lifetime Imaging Microscopy
FLIM- Fluorescence Lifetime Imaging Microscopy
Leica TCS SP5 AOBS (inverted) TCSPC (Time Correlated Single Photon Counting) FLIM
- FRET- Förster Resonance Energy Transfer
FRET- Förster Resonance Energy Transfer
Förster Resonance Energy Transfer (FRET) is a process where an excited fluorophore (donor)transfers energy to another fluorophore (acceptor) [1]. Reso...
- Brightfield & other Transmission Microscopy Contrasts
Brightfield & other Transmission Microscopy Contrasts
Brightfield illumination has been one of the most widely used observation modes in optical microscopy for the past 300 years. The technique is best su...
- High Speed Laser Scanning Confocal Systems
High Speed Laser Scanning Confocal Systems
Resonant Scanner
- Laser Scanning Confocal Microscopy
Laser Scanning Confocal Microscopy
Confocal microscopy offers several advantages over conventional widefield optical microscopy, including the ability to control depth of field, elimina...
- Live Cell Imaging
Live Cell Imaging
Cell biologists are increasingly using live-cell imaging techniques to provide clues into the fundamental nature of cellular and tissue structure and...
- Image Analysis/ Software
Image Analysis/ Software
An image processing workstation, equipped whith Imaris, SymphoTime and other image processing and analysis programs.
- Photobleaching Techniques/ FRAP-Fluorescence Recovery After Photoble...
Photobleaching Techniques/ FRAP-Fluorescence Recovery After...
Photobleaching techniques are used as a strategy to reveal the diffusion/binding dynamics of molecules or the exchange between compartments in live ce...
- Photoconversion
Photoconversion
Photoactivatable fluorescent proteins (PAFPs) exhibit fluorescence that can be modified by a light-induced chemical reaction. Many PAFPs have been eng...