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Olympus FV1000 / Olympus FV1000

Olympus FV1000 Total Internal Reflection Microscopy (TIRF)

Description

Inverted spectral confocal microscope equipped with 6 excitation lines, 4 detection channels (3 fluorescence/reflection, 1 transmitted light). An in vivo chamber can be attached.

Lasers: Diode UV 405 nm (50 mV)

Argon 458/488/515 nm (30 mV)

Diode 560 nm (20 mV)

Diode 635 nm (20 mV)

Fluorescence filters: Excitation UV BP 330-385, emission LP 420

Excitation BP 460-490, emission SP 520

Excitation BP 510-550, emission LP 510-550

Detectors: 3 PMT: 2 spectral detectors + 1 detection channel with filter

Objectives: UPLSAPO10x / NA 0.40

UPLSAPO20x / NA 0.75

UPLFLN40xO / NA 1.3 oil

UPLSAPO60xO / NA 1.35 oil

PLAPON60xO / NA 1.45 oil (TIRF)

Optics: Bright field

Nomarski

Phase contrast

TIRF illumination module + CellM software

In vivo system: In vivo chamber (PeCon)

Temperature controller

CO2 controller

Thermostatic plate