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Leica Microsystems Laser Scanning Confocal TCS-SP5 / Leica Laser Scanning Confocal TCS-SP5

Leica Microsystems Laser Scanning Confocal TCS-SP5 Multiphoton Microscope

Description

Inverted spectral confocal microscope equipped with 9 excitation lines, 6 detection channels (5 fluorescence/reflection, 1 transmitted light), with an AOBS® (Acousto Optical Beam Splitter) system. In vivo incubator and a fast scanning module (resonant scanner).

Lasers: Diode UV 405 nm (50mV)

Argon 458/476/488/496/514 nm

DPSS 561 nm

Helium-Neon 594 nm

Helium-Neon 633 nm

Fluorescence filters: Excitation UV BP 340-380, emission LP 425

Excitation BP 405-490, emission LP 515

Excitation BP 515-560, emission LP 590

Detectors: 3 PMT + 1 HyD (hybrid detection system for photon counting)

Objectives: HC PL APO CS 10x/0.40

HC PL APO CS 20x/0.70

PL APO 40x/1.25-0.75 CS oil

PL APO 63x/1.4-0.6 CS oil

PL APO 63x/1.2 W

PL APO 63x/1.3 Corr CS glicerol

HC PL Fluorar 50x/0.80 dry

Optics: Bright field

Nomarski

Polarized

In vivo system: Microscope incubator (Life Imaging Services)

Temperature controller

CO2 controller

Thermostatic and motorized/galvanometric plate

MP: Titanium-Sapphire IR MaiTai broadband laser 710-990nm

2 external detectors for reflected light (NDD)