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Leica Microsystems TCS SP8 / Leica TCS SP8

Leica Microsystems TCS SP8 Laser Scanning Confocal Microscope

Description

Inverted microscope Leica DMI6000CS

Touchscreen STP6000 Metal halide lamp EL6000 (120 W)

Fluorescence cubes: Type Light Excitation Dichroic Emission A4 UV BP 360/40 400 BP 470/40 CFP violet/blue BP 436/20 455 BP 480/40 I3 blue BP 450-490 510 LP 515 N2.1 green BP 515-560 580 LP 590

Objectives: Type Magnification/NA Technique Immersion PL APO CS 10x/0.4 PL APO CS 20x/0.70 PL APO CS2 20x/0.75 Nomarski water, glycerol, oil PL APO CS2 40x/1.1 Nomarski water PL APO CS2 63x/1.4 Nomarski oil

Ultraspectral confocal module Leica TCS SP8

FOV scanning: speed up to 1.800 Hz (3.600 Hz in bidirectional mode), 7 fps @ 512 x 512 px, resolution up to 8.192 x 8.192 px. 4 spectral detectors: 2 PMT and 2 HyD

1 detector for transmitted light

LIAchroic beam splitter, which includes the dichroic mirrors: substrate (405) 488/561/633 458/514/561 476/488 RT 15/85 (reflection) 488/561

Lasers: CW (405 nm). 50 mW Ar (458, 488 and 514 nm). 100 mW DPSS (561 nm). 20 mW. He-Ne (633nm). 10 mW

Software LAS AF SP8 microlab with wizard for FRET & FRAP analysis