Red Española de Microscopía Óptica Avanzada
FCS- Fluorescence Correlation Spectroscopy
 
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Description
 
Fluorescence Correlation Spectroscopy (FCS) is a tool that provides quantitative localized measurements of important physical parameters including mechanisms of transport, molecular mobilities, and densities of fluorescently labeled species. In the most basic configuration, FCS examines the inherent correlations exhibited by the fluctuating fluorescent signal from labeled molecules as they transition into and out of a specified excitation volume or area. The dual-color variation, termed Fluorescence Cross-Correlation Spectroscopy (FCCS), is utilized to probe two species labeled with different fluorophores. FCCS can extend investigations to the examination of biochemical reactions between two partners, such as reaction rates, kinetics, fractions of binding or reacting molecules, and mobilities of a complex formed between the partners.
This technique at work
 
Centre for Genomic Regulation, Parc de Recerca Biomèdica Barcelona - Advanced Light Microscopy Unit
 
Instituto de Investigacion Biomedica Sant Pau, Instituto de Investigacion Sant Pau - Plataforma Cientifico-Tecnica de Microscopia Confocal
 
Universidad Complutense de Madrid, Centros de Asistencia a la Investigacion (CAIS-ICTS) - Flow Cytometry and Fluorescence Microscopy Unit, UCM
 
Consejo Superior de Investigaciones Cientificas (CSIC), Centro Nacional de Biotecnologia (CNB-CSIC) - Servicio de Microscopia Optica Avanzada del Centro Nacional de Biotecnologia
 
Consejo Superior de Investigaciones Cientificas (CSIC), Instituto Biofisika (CSIC-UPV/EHU) - Basque Resource for Advanced Light Microscopy
 
IRB Barcelona (Institute for Research in Biomedicine), IRB Barcelona (Institute for Research in Biomedicine) - Advanced Digital Microscopy Core Facility (IRB Barcelona)
 
CIC biomaGUNE, CIC biomaGUNE - Optical spectroscopy and microscopy plaftorm
 
 
Related training
 
 
Type Document Dates Lab
 
Related material
 
 
Type Details Title Lab
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